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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study does not indicate if the substance was toxic to microbes.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
no
Remarks:
The study was conducted according to the test guidelines in effect at the time of study conduct.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Purity: R7623 Technical, solid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
0 - 28 d
Initial test substance concentrationopen allclose all
Initial conc.:
2 µg/L
Based on:
test mat.
Initial conc.:
10 mg/L
Based on:
test mat.

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Remarks on result:
other: result for 2 mg/L concentration
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Remarks on result:
other: result for 10 mg/L concentration

Any other information on results incl. tables

After 28 days the reference substance was degraded more than 60% thereby validating the method.

The theoretical oxygen demand (ThOD) was determined to be 1.777 mg O2/mg test substance.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The test substance appeared to be not biodegradable in the closed bottle test.
Executive summary:

A closed bottle biodegradation test was conducted with two concentrations of the test substance according to OECD Guideline 301 D. Samples were incubated in the dark at approximately 20ºC and analyzed at 5 day intervals to determine the biological oxygen demand. There was no degradation after 28 days, indicating that the test substance is not readily biodegradable.