2,2',4'-trichloroacetophenone

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study according to international accepted guidelines and GLP compliant.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,2',4'-trichloroacetophenone

EC Number:

224-218-2

EC Name:

2,2',4'-trichloroacetophenone

Cas Number:

4252-78-2

Molecular formula:

C8H5Cl3O

IUPAC Name:

2-chloro-1-(2,4-dichlorophenyl)ethan-1-one

Test material form:

solid: crystalline

Details on test material:

Test item: 2,4,ω-Trichloroacetophenone (CAS No. 4252-78-2)
Molecular formula: C8H5Cl3O
Molecular weight: 223,5 g/mol
Colour: white
Purity: ~ 97 %
Storage temperature: 20 °C, keep it in dark place

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

static

Total exposure duration:

96 h

Test conditions

Test temperature:

21,4 - 22, 4 °C
Control: 21,0 - 22,8 °C

pH:

7,2 - 8,5
Control: 8,4 - 7,8

Dissolved oxygen:

4,1 - 9,3 mg/l
Control: 6,3 - 9,5

Nominal and measured concentrations:

0 mg/l
0,5 mg/l
1 mg/l
2,2 mg/l

Details on test conditions:

Feeding: twice a day
Animal density: ≤ 1 g fish / L water
It was used 10 fish for each concentrationen.
The fish weren't fed under the test.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.5 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.5 - 1 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

2.2 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Conclusions:

In this Acute Toxicity Test of "Trichloroacetophenone" (CAS No. 4252-78-2) the results indicated that the test item is hazardous to the aquatic environment [GHS Acute aquatic hazard Category Acute I. – 96 hr EC50 (for fish) ≤ 1 mg/L.