Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

A weight of evidence approach is discussed.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.01 mg/L

Additional information

Long-term toxicity of tris(methylphenyl)phosphate was assessed in an early-life stage toxicity test with Jordanella floridae. The experimental procedure was based on the dutch national standard method NEN 6504: Acute toxicity test with Poecilia reticulata. The early-life stages of fish are exposed for 28 days to 5 concentrations of the test substance dissolved in water, first under static conditions, and after hatching under semi-static conditions. The test begun by placing fertilised eggs in the test chambers and is continued at least until all the fish are free-feeding. Lethal effects and quantitative growth (weight and length) as sub-lethal effects are assessed and compared with control values to determine the no observed effect concentration (NOLC/NOEC). Stability of test substance was monitored during the experiment. All results refer to measured concentration. Embryonic development took place for 5 to 6 days and within one day all fish hatched. It was shown that hatched fish are more sensitive than embryos (eggs). For the endpoint egg development, a NOEC of 1.0 mg/l was obtained. Observation on mortality and sub-lethal effects results in a NOLC and NOEC of 0.01 mg/l. As analytical monitoring was carried out, the results relate to measured concentrations. This toxicity study is classified as acceptable and satisfies generally accepted scientific standards for the early-life stage toxicity test.

3 additional studies are also included. Although limited data is reported within these, the results are considered to support a weight of evidence approach, as these are comparable to other values for long term toxicity to fish in other studies, including those on analogues. 

A NOEC for long term fish toxicity is assigned as 0.01 mg/l