1,1,3-trichloroacetone

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-03-04 to 2002-09-18

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,1,3-trichloroacetone

EC Number:

213-063-6

EC Name:

1,1,3-trichloroacetone

Cas Number:

921-03-9

Molecular formula:

C3H3Cl3O

IUPAC Name:

1,1,3-trichloropropan-2-one

Details on test material:

- Name of test material (as cited in study report): 1,1,3-Trichloraceton
- Substance type: Industrial chemical
- Physical state: liquid
- Purity test date: not given in report
- Lot/batch No.: 1TC112013
- Expiration date of the lot/batch: 2002-12-31
- Stability under test conditions: stable
- Storage condition of test material: room temperature in the dark

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES

- Stability of test material at room temperature: stable
- pH dependance on stability: stable

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The inoculum used was a secondary effluent from a public waste water treatment plant in Plönm, Germany.
- Storage conditions: kept aerobic
- Storage length: used on day of collection
- Pretreatment: filtered, the first 200 ml beeing discarged
- Initial cell/biomass concentration: 2 X 10e8 bacteria
- Water filtered: yes
- Type and size of filter used, if any:coarse filter

Duration of test (contact time):

28

Initial test substance concentration

Initial conc.:

4 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: 0.85 g KH2PO4; 2.85 g K2HPO4 x 3H2O; 3.34 g Na2HPO4 x 2 H2O; 0.05 g NH4Cl,; 2.25 g MgSO4 x 7H2O; 3.64 g CaCl2 x 2 H2O; 0.025 g FeCl3 x g H2O in 100 ml; 1 ml of this solution isadded to 1 l of destilled water
- Test temperature: 19.8 °C
- pH: not given
- pH adjusted: no
- Aeration of dilution water: yes
- Continuous darkness: yes


TEST SYSTEM
- Number of culture flasks/concentration: 18 bottles
- Method used to create aerobic conditions: aeration
- Measuring equipment: Oxi DIGI 530 with a Tri Oxmatic EO 200 oxygen electrode
- Test performed in closed vessels due to significant volatility of test substance: yes
- Test performed in open system: no


CONTROL AND BLANK SYSTEM
- Inoculum blank: 18 bottles
- Abiotic sterile control: 18 bottles
- Toxicity control: 6 bottles

Results and discussion

Preliminary study:

not performed

Test performance:

as stated in the Guideline

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

4

Sampling time:

3 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

9.8

Sampling time:

10 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

13.4

Sampling time:

14 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

4

Sampling time:

21 d

% Degradation 5

Parameter:

% degradation (O2 consumption)

Value:

5

Sampling time:

28 d

Details on results:

not available

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

Test substance is not readily biodegradable under conditions used.

Executive summary:

1,1,3 -Trichlorocetone was examined for its redily biodegradability in a 28 days closed bottle test according to OECD Guideline 301 D at a concentration of 4 mg/l. After 28 days a biodegradation of 5.0 % could be observed. Therefore 1,1,3 -Trichloroacetone was only slightly biodegradable over the 28 day exposure period to a secondary effluent from a domestic sewage treatment plant. The criterion for ready biodegradability (at least 60 % biodegradation within ten days of biodegradation exceeding 10 %) was not reached. On the other hand, the reference compound sodium acetate was biodegraded by an average of 75.3% by exposure day 14 and reached an average biodegradation rate of 84.9% by the end of the 28 day exposure period, this confirming suitability of the secondary effluent. The test substance did not inhibit the inoculum in the toxicity control.

In conclusion, 1,1,3 -Trichloroacetone was not readily biodegradable under the conditions of the Closed Bottle test presently performed.