Registration Dossier
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EC number: 629-764-9 | CAS number: 164524-02-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 26-30, 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study without GLP or analytical confirmation
- Justification for type of information:
- Please see Category Approach
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 797.1050 (Algal Toxicity, Tiers I and II)
- Deviations:
- no
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Strain 1648, class Chlorophyceae originally obtained from Carolina Biological Supply Company; source is SLI culture
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 24-25 degrees Celcius
- pH:
- 7.5 at initiation. 9.6-10 at 96 hours
- Nominal and measured concentrations:
- 0, 16, 31, 63, 130, 250, 500, 1000 mg/L active ingredient (nominal)
- Details on test conditions:
- TEST SYSTEM- Test vessel: - Type (delete if not applicable): closed - Material, size, headspace, fill volume: 125-mL Erlenmeyer flasks with 50 mL of test solution - Aeration: shaking table set at 100+/-10 rpm- Type of flow-through (e.g. peristaltic or proportional diluter): - Renewal rate of test solution (frequency/flow rate):- Initial cells density: 67 x 10+4 cells/mL (inoculated with 0.75 mL)- Control end cells density: - No. of organisms per vessel:- No. of vessels per concentration (replicates): 3- No. of vessels per control (replicates): 3- No. of vessels per vehicle control (replicates):GROWTH MEDIUM- Standard medium used: yes (AAP medium)- Detailed composition if non-standard medium was used:TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water:- Total organic carbon:- Particulate matter:- Metals:- Pesticides:- Chlorine:- Alkalinity:- Ca/mg ratio:- Conductivity:- Culture medium different from test medium:- Intervals of water quality measurement: OTHER TEST CONDITIONS- Sterile test conditions: yes- Adjustment of pH:- Photoperiod: 24 hr light- Light intensity and quality: 350-425 footcandles and 3800-4600 lux. Duro-Test, Inc. Vita-Life fluorescent lights- Salinity (for marine algae):EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : - Determination of cell concentrations: [counting chamber - hemacytometer (Neubaure improved)- Chlorophyll measurement:- Other: TEST CONCENTRATIONS- Spacing factor for test concentrations: 2X- Justification for using less concentrations than requested by guideline: - Range finding study - Test concentrations: 0.010 to 1000 mg/L - Results used to determine the conditions for the definitive study: controls 104 and exposed ranged from 100 to 44 x10+4 cells/mL
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 230 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- cell number
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 31 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- cell number
- Details on results:
- At test termination, cell fragments and bloated cells were observed in the 500 and 1000 mg A.I./L treatment levels while thin cells were observed in the 63, 130 and 250 mg A.I./L treatment levels. Normal algal cells were observed in the 16 and 31 mgA.I./L treatment levels and in the controls. The control cultures averaged 102 x 10+4 cells/mL at termination. Cell densities at 16, 31, 63, 130, 250, 500 and 1000 mg A.I./L averaged 96, 97, 76, 69, 59, 29 and 11 x 10+4 cells/mL at termination. Statistical analysis (William's test) demonstrated a significant reduction in cell density in the 63 mgA.I./L and higher treatment levels. The 96-hour no observed effect concentration for cell density was determined to be 31 mg A.I./L. The 96-hur EC10 and EC90 values were calculated to be 41 and 1300 mg A.I./L, respectively. No test concentration completely inhibited algal growth. A subsample of the composite of three replicates of the highest test concentration was diluted with fresh AAP medium for the determination of growth recovery. After six days in fresh medium the 1.6 x 10+4 cells/mL became 127 x 10+4 cells/mL. Therefore, it is concluded that the test substance has an algistatic rather than algicidal effect at 1000 mg A.I./L.
- Reported statistics and error estimates:
- The 95% confidence limits of the 96-hr EC50 are 47 to 1200 mg A.I./L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance has an algistatic rather than algicidal effect at 1000 mg A.I./L.; the EC50 is 230 mg A.I./L
Reference
Description of key information
The key study reported a 96-hour EC50 of >= 230 mg/L active substance based on Selenastrum capricornutum (new name: Pseudokirchnerella subcapitata) cell density (with a 95% CL of 47 to 1200 mg/L).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 230 mg/L
- EC10 or NOEC for freshwater algae:
- 31 mg/L
Additional information
The key study is a reliable 1993 USEPA Guideline study (EPA OTS 797.1500 - Algal Toxicity). The freshwater Selenastrum sp. was exposed to a range of nominal concentrations of the test substance, sodium xylenesulphonate that reported a 96 -hour EC50 of >= 230 mg/L active substance based on cell density (with a 95% CL of 47 to 1200 mg/L). There was an apparent dose response and the 96-hour NOEC was 31 mg/L. The test substance was observed to be algistatic but not algicidal at the highest concentration tested (1000 mg/L active substance).
Two supporting studies were available. A 1994 EPA Guideline study (EPA OTS 797.1500) with the test substance calcium xylenesulphonate was conducted under GLP requirements and with analytical confirmation reported a 96 -hour EC50 of 758 mg/L active substance (with a 95% confidence limit of 592-924 mg/L) and a NOEC of 240 mg/L based on growth rate of Selenastrum sp. A 1995 EU Guideline study (EU C.3 Algal Inhibition Test) of sodium toluenesulphonate conducted under GLP requirements and with analytical confirmation reported a 72 -hour EbC50 of 150 mg/L active substance based on cell density and a 72 -hour ErC50 of 310 mg/L based on specific growth rate of Scenedesmus sp. The 72 -hour NOEC is 40 mg/L active substance based on biomass.
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