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Toxicological information

Repeated dose toxicity: inhalation

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Administrative data

Endpoint:
short-term repeated dose toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The report was very limited documented, although the analytical part was more extensive. Also limited parameters were observed.

Data source

Reference
Reference Type:
publication
Title:
Ammonia nitrate inhalation test on rats
Author:
KemaMord
Year:
1978
Bibliographic source:
Research Institute of Swedish National Defense (FOA) and the National Veterinary Institute (SVA), Stockholm, Sweden (cited in European Commission 2000).

Materials and methods

Principles of method if other than guideline:
No data
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ammonium nitrate
EC Number:
229-347-8
EC Name:
Ammonium nitrate
Cas Number:
6484-52-2
Molecular formula:
H3N.HNO3
IUPAC Name:
ammonium nitrate
Test material form:
solid
Remarks:
crystals or granules
Details on test material:
ammonium nitrate dust collected from filters used in the ventilation unit at the KemaNord ammonium nitrate factory at Ljungaverk

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male

Administration / exposure

Route of administration:
inhalation
Type of inhalation exposure:
nose/head only
Vehicle:
other: no data
Remarks on MMAD:
MMAD / GSD: particles < 5 microm. = between 26 and 50 wt%.
30% of the particles were < 10 micrometer
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
2 weeks
Frequency of treatment:
5 hrs/day; 5 days/week
Doses / concentrations
Dose / conc.:
185 mg/m³ air (analytical)
Remarks:
Maximum dose
Minimum dose: 26 mg/m^3 air
No. of animals per sex per dose:
10 animals were exposed to ammonium nitrate
Control animals:
other: yes, 5 control animals were exposed to an air current, 5 control animals were totally untreated.

Examinations

Observations and examinations performed and frequency:
Bodyweights were registered before, during and after exposure.
Sacrifice and pathology:
After killing, autopsy was carried out and organs were taken for histopathological analysis.

Results and discussion

Results of examinations

Clinical signs:
no effects observed
Mortality:
no mortality observed
Body weight and weight changes:
no effects observed
Gross pathological findings:
no effects observed
Histopathological findings: non-neoplastic:
no effects observed

Effect levels

Key result
Dose descriptor:
NOAEC
Remarks:
systemic
Effect level:
>= 185 mg/m³ air
Sex:
male
Basis for effect level:
other: No effects observed.

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

A certain irritation in the nostrils of the test group was seen, no further symptoms. However, it was not clear at which concentrations these effects were seen. No significant differences in body weight between test and control animals. No macroscopically or microscopically effects have been seen. Post exposure observation period: 3 days

Applicant's summary and conclusion

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