Registration Dossier
Registration Dossier
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EC number: 229-347-8 | CAS number: 6484-52-2
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Not a standard protocol
Data source
Reference
- Reference Type:
- publication
- Title:
- Tolerance of Bullia digitalis (Prosobranchiata) to solutions of ammonium nitrate in natural seawater.
- Author:
- Brown, A.C. and Currie, A.B.
- Year:
- 1 973
- Bibliographic source:
- So. Afr. Journ. Sci. 69:219-220
Materials and methods
- Principles of method if other than guideline:
- No data
- GLP compliance:
- no
Test material
- Reference substance name:
- Ammonium nitrate
- EC Number:
- 229-347-8
- EC Name:
- Ammonium nitrate
- Cas Number:
- 6484-52-2
- Molecular formula:
- H3N.HNO3
- IUPAC Name:
- ammonium nitrate
- Test material form:
- solid
- Remarks:
- granules or crystals
- Details on test material:
- No further details provided.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- other: Bullia digitalis (prosobranch gastropod)
Study design
- Test type:
- static
- Water media type:
- saltwater
- Total exposure duration:
- 7 d
- Remarks on exposure duration:
- Up to 7 days
Test conditions
- Test temperature:
- 12.5 ± 0.5 °C
- Nominal and measured concentrations:
- Nominal concentrations: 0, 25, 50, 100, 300, 500, 1000 and 1500 ppm
Results and discussion
Effect concentrations
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 555 mg/L
Any other information on results incl. tables
No mortality occurred even at the highest concentration (1500 ppm) during the 7 day study period. No significant treatment related effects were observed below 300 ppm, although animals at concentrations between 100 and 300 ppm showed slight transient signs of stress from which they recovered spontaneously within 3 days. At between 300 and 400 ppm, most individuals showed signs of stress within 6 hours and many eventually became paralyzed. At concentrations of 500 ppm and higher, the paralysis was more marked and permanent, with the time to onset decreasing as concentration increased.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
