2-[2-(2-ethoxyethoxy)ethoxy]ethyl methacrylate

Endpoint summary

Additional information

Freshwater aquatic toxicity studies with Ethyltriglycol methacrylate were conducted in fish, daphnia, algal and microbial species.

Short-term toxicity to fish

One acute freshwater study in fish is available for Ethyltriglycol methacrylate, which is considered reliable with restriction (Klimisch score 2).

In a 48-h acute toxicity study according to DIN 38412, part 15, Golden orfe (Leuciscus idus melanotus) were exposed to Ethyltriglycol methacrylate under static conditions.  

The 48-h EC₅₀was 406 mg/L (95% CL (confidence limit): 230 - 715 mg/L). Based on the results of this study, Ethyltriglycol methacrylate would be classified as non toxic to Leuciscus idus melanotus (fish) in accordance with the classification system of the EU-GHS.

Short-term toxicity to aquatic invertebrates

There are no short-term data available for aquatic invertebrates with Ethyltriglycol methacrylate but there is a long-term toxicity study to daphnia (21-day study, semi-static conditions according to OECD 211, Klimisch score 1, Evonik Industries AG, 2012).

In this vaild long-term guideline study for aquatic invertebrates (daphnids) Ethyltriglycol methacrylate has a NOEC of 100 mg/L (21-day, daphnia magna) based on mortality of adult daphnids. Therefore the short-term toxicity to daphnids EC50 (48 h) for Ethyltriglycol methacrylate is expected to be greater than 100 mg/L (based on mortality). For this reason there is no reason to perform a short-term toxicity test on aquatic invertebrates.

Toxicity to aquatic algae

One study in freshwater algae is available for Ethyltriglycol methacrylate, which is considered reliable without restriction (Klimisch score 1).

In a 72 hour acute toxicity study (static dose-response test), the cultures of Pseudokirchneriella subcapitata strain no. 61.81 SAG were exposed to Ethyltriglycol methacrylate at nominal concentrations of 0, 6.25, 12.5, 25, 50, and 100 mg/L under static conditions in accordance with the guideline OECD 201. The ErC₅₀ value and the

ErC₁₀ value based on growth rate was > 100 mg/L and EbC₅₀ value based on yield was > 100 mg/L after 72 hours, respectively. The NOEC was 12.5 mg/L (72 h, growth rate and yield) and LOEC was 25 mg/L (72 h, growth rate and yield).

Long-term toxicity to fish

Long-term testing in fish is waived for Ethyltriglycol methacrylate since the substance is readily biodegradable. The risk characterisation shows that the PEC/PNECaqua ratio for the aquatic environment is <1, indicating no need for further information or testing. According to REACH regulationAnnex IX, 9.1. column 2, long-term toxicity testing shall only be considered when the chemical safety assessment indicates the need for further investigations. Thus, no long-term toxicity testing is required for Ethyltriglycol methacrylate.

 

Long-term toxicity to aquatic invertebrates

In a valid guideline study (OECD 211, semi-static conditions), the 21 day reproductive toxicity to daphnids was determined to be EC50: > 100 mg/L for Daphnia magna (STRAUS Clone 5), NOEC: 32 mg/L for the reproduction and 100 mg/L for the survival of the adult test animals. The EC10 21 d to daphnia magna (reproduction) of the substance Ethyltriglycol methacrylate was 77.1 mg/l. All effect values given are based on the geometric mean

measured test concentrations of Ethyltriglycol methacrylate. (Klimisch score: 1, Evonik Industries, 2013)

Toxicity to microorganisms

In accordance with column 2 of REACH Annex VIII, the activated sludge respiration inhibition test does not need to be conducted as this substance was found to be readily biodegradable in an OECD 301 F Manometric respiration test at an application concentration of 100 mg/L. There was no evidence for toxicity to bacteria. (Klimisch score : 2, for details of the study please see chapter: 5.2.1, Evonik Industries 1999)

Furthermore the toxicity of Ethyltriglycol methacrylate to microorganisms (Pseudomonas putida) was evaluated in bacteria toxicity test according to Bringmann-Kühn (modified DIN 38412, part 8) with limited documentation (Klimisch score: 4). The 16h-EC10 in this study was determined to be > 8000 mg/L for Ethyltriglycol methacrylate (for details of the study please see chapter: 6.1.7, Evonik Industries 1988).