Melamine

Repeated dose toxicity: other routes

Administrative data

Endpoint:

repeated dose toxicity: other route

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Study with another goal and only by the way reporting of results for melamine.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

The nitrogen mustard-like action of 2,4,6-tris(ethylen-imino)-s-triazine and other bis(ethyleneimines)

Author:

Philips FS and Thiersch JB

Year:

1950

Bibliographic source:

Pharmacol. Exp. Ther., 100, 398-407, (1950)

Materials and methods

Principles of method if other than guideline:

Toxicity in rats and mice after 5 intraperitoneal doses of melamine.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Melamine

EC Number:

203-615-4

EC Name:

Melamine

Cas Number:

108-78-1

Molecular formula:

C3H6N6

IUPAC Name:

1,3,5-triazine-2,4,6-triamine

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Duration of treatment / exposure:

5 days

Frequency of treatment:

daily

Doses / concentrations

Remarks:

Doses / Concentrations: 500 mg/kg

Control animals:

yes, concurrent no treatment

Results and discussion

Effect levels

Dose descriptor:

other: LD50

Effect level:

> 500 mg/kg bw/day

Target system / organ toxicity

Critical effects observed:

yes

Lowest effective dose / conc.:

500 mg/kg bw/day (nominal)

System:

other: body weight and kidney

Organ:

kidney

Treatment related:

yes

Dose response relationship:

no

Relevant for humans:

no

Any other information on results incl. tables

There was a moderate weight loss. In two animals crystalline deposits were found in renal tubules. The toxic effects of several s-triazines with nitrogen-mustard like action were compared to the effects of melamine. Melamine did not induce nitrogen-mustard like actions on peripheral blood or bone marrow. No intestinal lesions could be found in animals treated with melamine. 

Mice tolerated five successive daily doses of melamine (500 mg/kg; i.p.). 

No further results are presented.

Applicant's summary and conclusion

Executive summary:

The toxic effects of several s-triazines with nitrogen-mustard like action were compared to the effects of melamine. Melamine did not induce nitrogen-mustard like actions on peripheral blood or bone marrow. There was a moderate weight loss. In two animals crystalline deposits were found in renal tubules. No intestinal lesions could be found in animals treated with melamine. Mice tolerated five successive daily doses of melamine (500 mg/kg; i.p.).