2,6-di-tert-butyl-p-cresol

Endocrine disrupter testing in aquatic vertebrates – in vivo

Administrative data

Endpoint:

fish: other

Remarks:

fish estrogen receptor.

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1995

Reliability:

4 (not assignable)

Data source

Reference

Reference Type:

publication

Title:

A Variety of Environmentally Persistent Chemicals, Including Some Phthalate Plasticizers, Are Weakly Estrogenic

Author:

Jobling, S. et. al.

Year:

1995

Bibliographic source:

Environmental Health Perspectives, Volume 103, Number 6, June 1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Direct binding of the chemical to the fish estrogen receptor

Deviations:

not applicable

Principles of method if other than guideline:

Because of their documented presence in the aquatic environment, the initial examination for estrogenicity was carried out by measuring direct binding of the chemicals to the fish estrogen receptor.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2,6-di-tert-butyl-p-cresol

EC Number:

204-881-4

EC Name:

2,6-di-tert-butyl-p-cresol

Cas Number:

128-37-0

Molecular formula:

C15H24O

IUPAC Name:

2,6-di-tert-butyl-4-methylphenol

Test material form:

solid

Test organisms

Aquatic vertebrate type:

fish

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

The initial screening process was carried out using a cytosolic extract from the liver. Estradiol receptor-binding sites are present here in both male and female fish.

Study design

Total exposure duration:

1 h

Test conditions

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

1 h

Dose descriptor:

NOEC

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: stimulation of endocrine receptor activity

Remarks:

liver

Any other information on results incl. tables

At this high concentration (22 mg/L) the stimulation of the endocrine receptor (ER) was, however, less than 15 % of the maximum response obtained with 17ß-estradiol. It is reported that BHT did not “impair binding of tritiated estradiol to the ER”. Thus, there was no proof for an interaction of BHT with the ER receptor.

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

No stimulation of the receptor activity was determined for BHT until concentrations exceeded a concentration of 1E-4 M (= 22 mg/L).

Executive summary:

Jobling et al. used a fish endocrine receptor (ER) binding assay with Oncorhynchus mykiss cells. The initial screening process was carried out using a cytosolic extract from the liver. No stimulation of the receptor activity was determined for BHT until concentrations exceeded a concentration of 1*10^-4 M (22 mg/L). At this high concentration the stimulation of the ER was, however, less than 15 % of the maximum response obtained with 17ß-estradiol. Jobling et al. additionally reported that BHT did not “impair binding of tritiated estradiol to the ER”. Thus, there was no proof for an interaction of BHT with the ER receptor.