Registration Dossier
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EC number: 231-302-2 | CAS number: 7488-55-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Oxidation reduction potential
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- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
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- Genetic toxicity
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- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Neurotoxicity
Administrative data
- Endpoint:
- neurotoxicity
- Remarks:
- other: acute experiment in ganglion of mollusk
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: no glp, no guideline, but well documented
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Methods
The effects of SnCl_2 and Sn8CH_3)_2 on the acetylcholine-activated currents were studied in
identified neurons of the pulmonate mollusk Lymnaea stagnalis, using a two microelectrode
membrane patential clamping method and intracellular dialysis in conditions of fixed potential
and ion concentrations.
Adult animals were colleceted in Lake Kish-Balaton and kept in aquarium at a temperature of
20-22°C. Experiments were conducted either on neurons in whole gaglion preperations or after
isolation of induvidula nerve cells.
Ganglia were prepared and identified neurons were isolated. Experiments using the two micro-
electrode membrane potential clamping technique, dissected ganglia were incubated in physiological
saline containing 0.1% protease for 5 min at room temperature. Connective tissue was then
removed and ganglia were washed in physiological saline and stored in the refigerator at 4°C
for 1 -2 h.
Experiments were performed using identified neurons RPcD1, VV12, VDC and RPaD1. Standard solution
contained 44mM NaCl, 1.7 mM KCl, 4 mM CaCl_2, 10 mM HEPES, pH 7.4. Solution for intracellular
dialysis consists of 43.5 mM KOH, 20 mM KCl, 10 mM EGTA, 2.5 mM HEPES, pH 7.3. This membrane
potential clamping method used a Dagan 8500 amplifier and an Axopatch-1D amplifier. Two
independent mircolecetrodes filled with 2.5 M potassium acetate and 25 mM KCl were insered in
neurons. Potential steps and data analyis were carried out using an A/D-D/A concerter card and
a standalone program. Chemical compounds were obtained from Sigma and Reanal. All experiments
were conduceted at room temperature (20-22°C). Acetylcholine was applied by mircoperfusion or
by replacing the whole solution for 12-16 sec with intervals 4-5 m in. - GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Tin dichloride
- EC Number:
- 231-868-0
- EC Name:
- Tin dichloride
- Cas Number:
- 7772-99-8
- Test material form:
- solid - liquid: suspension
- Details on test material:
- -
Constituent 1
Test animals
- Species:
- other: mollusk
- Strain:
- other: Lymnaea stagnalis
- Sex:
- not specified
Administration / exposure
- Route of administration:
- other: not relevant, see method
- Vehicle:
- other: not relevant, see method
- Analytical verification of doses or concentrations:
- not specified
- Frequency of treatment:
- see method
Doses / concentrations
- Remarks:
- Doses / Concentrations:
see method
Basis:
other: see method
- No. of animals per sex per dose:
- see method
- Control animals:
- yes
Results and discussion
Effect levels
- Dose descriptor:
- other: inhibition of sodium channels
- Effect level:
- 25 other: µM
- Based on:
- test mat.
- Sex:
- not specified
- Remarks on result:
- other:
Any other information on results incl. tables
see publication
Applicant's summary and conclusion
- Conclusions:
- Sn+2 inhibits reversible the sodiom channels in ganglion
- Executive summary:
In isolated single neurone or in whole ganglia preparation, SnCl2 caused a decrease in the acetylcholine-induced inward current in a dose-dependent way. Threshold concentration was 0.1 mkM and saturated at 5 mkM SnCl2. After 10-min. pre-treatment with SnCl2 the effect was more obvious (by 20%) than after 3-min pre-treatment. Sn(CH3)2 decreased the amplitude of Ach-induced current similarly to the inorganic Sn. The effect of Sn(CH3)2 was irreversible. The findings support earlier data that the agonist-activated channels are important targets for toxic metals. Direct action upon neuronal membrane seems to be an important event in modulation of synaptic transmission.
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