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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012-02-15 till 2012-03-22
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline-conform study under GLP without deviations.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
1050, 583, 324, 180 und 100 mg test substance/L and a control.
Test organisms (species):
Pimephales promelas
Details on test organisms:
Juvenile Fathead minnow (Pimephales promelas); mean length (± SD): 2.36 cm ± 0.30 cm; mean body weight (± SD): 0.11 g ± 0.04 g
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no data
Hardness:
Water Hardness: 2.5 mmol/L (= 250.0 mg/L) as CaCO3
Alkalinity: 0.8 mmol/L
Test temperature:
Water temperature: 22 to 23 °C
pH:
pH value: 7.8 to 8.2
Dissolved oxygen:
dissolved oxygen concentration: 98 to 99 % of the air saturation value
Salinity:
no
Nominal and measured concentrations:
At the start of the test just before introduction of the fish 95 % of the nominal test concentrations were found. After 96 hours test duration 97 % of the nominal values were determined (mean value of all concentrations at or above the NOEC determined in this test). Thus, during the test period of 96 hours the fish were exposed to a mean of 96 % of nominal. Therefore, all reported results are related to nominal concentrations of the test item.
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
1 141 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: n.d.
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
1 027 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: n.d.
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
583 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: n.d.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
934.1 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: n.d.
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 1 050 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: n.d.
Validity criteria fulfilled:
yes
Conclusions:
The active substance caused no mortality to Fathead minnow (Pimephales promelas) after 96-hours exposure at the nominal concentration of 583 mg/L in a static test, and a nominal 96h-LC50 of 934.1 mg/l could be determined.

Description of key information

The substance was not acute toxic to Fathead minnow (Pimephales promelas)  in a static aquatic freshwater toxicity test performed for 96 hours.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Remarks:
96h
Effect concentration:
934.1 mg/L

Additional information

The acute toxicity values for the active substance with Fathead minnow (Pimephales promelas) were calculated as following:

96h-LC50 = 934.1 mg /L (nominal)

96h-NOEC = 583 mg /L (nominal)