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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Description of key information

No toxicity observed even at highest concentration level tested.
RA_CAS115733-09-0_OECD 202_Daphnia magna: EC50 (48h) > 1000 mg/L WAF,
RA_CAS61789-86-4_OECD 202_Daphnia magna: EC50 (48h) > 100 mg/L WAF

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1 000 mg/L

Additional information

General considerations

For the target substance itself, no experimental data are available. The following read-across substances were used to fulfil the endpoint of short-term toxicity to Daphnia magna: Calcium sulfonate read-across substance (CAS 115733-09-0). Investigations concerning toxicity in marine invertebrates were not performed. For the detailed procedure of the read-across principle and justifications, please refer to the separate Read-Across Statement attached to this Chemical Safety Report.

Acute toxicity to freshwater invertebrates

Two key studies are available to characterise acute toxic effects of the test substance towards aquatic invertebrates. Based on the intrinsic properties of the used substances, Water Accommodated Fractions (WAFs) were prepared for testing. Daphnia magna was exposed to the test substance for a duration of 48 h, whereby mortality, immobilisation as well as sublethal effects were recorded daily.

In the first key study, the toxicity of the calcium sulfonate read-across substance (CAS 115733-09-0) under static conditions was investigated by Ward (1993) in accordance to US EPA Guideline OTS 797 -1300

. Daphnids were exposed to control and test chemical at nominal concentrations of 100, 300 and 1000 mg/L WAF for 48 hours. After 24 h, no immobilised or dead organisms were observed. At the end of the experiment, 5 %, 0 %, 20 % and 5 % immobilisation were reported for the control, 100, 300 and 1000 mg/L WAF, respectively. Therefore, the test substance is considered as non-toxic to daphnids at WAF loading rates up to 1000 mg/L. The EC50 (48h) is > 1000 mg/L WAF.

In the second key study, performed with Sulphonic acids, petroleum, calcium salts (CAS 61789-86-4), Daphnia magna were exposed also under static conditions for 48 hours (Goodband, 2005). Beside control, daphnids were exposed to a single nominal test substance concentration of 100 mg/L WAF for 48 hours. Immobilisation and sublethal effects were observed daily. No immobilisation was observed in any daphnids at 100 mg/L nominal WAF loading rate. The EL50(48h) is considered as > 100 mg/L.


No toxic effects observed in both acute studies with daphnids even at the highest concentration levels tested.