Alcohols, C12-14, ethoxylated, sulfates, sodium salts

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 - 15 Oct 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Initial biomass concentration: extinction of 0.140 at a wave length of 436 nm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Remarks on exposure duration:

16 +/- 1 hour

Test temperature:

21.8 °C

pH:

before incubation: 5.6 - 6.2
after incubation: 6.8

Nominal and measured concentrations:

nominal content of active substance: 313, 626, 1251, 2502.5, 5005 and 10010 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 mL Erlenmeyer flasks filled with 100 mL test medium
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

Reference substance (positive control):

no

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

Test Concentration [mg a.s./L]	Inhibition [%]
313	-6.9
626	-7.3
1251	-4.7
2502.5	-2.9
5005	-0.9
10010	7.4

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

23 Feb - 01 Mar 1993

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Does not meet important criteria of today standard methods

Qualifier:

according to guideline

Guideline:

other: DIN 38412 part 27

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Reference 3

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

not stated

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Only short abstract available

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Qualifier:

according to guideline

Guideline:

other: DIN 38412 part 12

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Pseudomonas putida

Test type:

static

Limit test:

no

Total exposure duration:

30 min

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

500 mg/L

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Description of key information

EC10 > 10 g/L based on growth inhibition

Additional information

One reliable study on microorganisms is available for AES (C12-14, 1-2.5EO) Na (CAS 68891-38-3). In the GLP-study (Sasol, 1993d) on growth inhibition of Pseudomonas putida according to DIN 38412, part 8 an EC10 and an EC50 -values of > 10 g/L could be determined after approximately 16 h of exposure. This study is defined as key study. The two additions study available for microorganisms (BASF, 1993b and BASF, 1998d) are regarded as not reliable as test duration and given endpoints based on respiration rate do not meet today standard methods.