Phthalic anhydride

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Principles of method if other than guideline:

method corresponds in most parts to OECD TG 209

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

activated sludge

Details on inoculum:

laboratory scale sewage treatment plant treating synthetic sewage (OECD 209)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Details on test conditions:

Type: aquatic

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Executive summary:

Toxicity of phthalic anhydride to activated sludge was evaluated by Bayer AG (1984) according to the ISO 8192 yielding an EC50 value of > 1000 mg/l when exposed to phthalic anhydride for 3 hours at a pH between 7.4 and 7.6. (Bayer, 1984)

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

other: ISO 10712, 1995 Pseudomonas putida growth inhibition test

Principles of method if other than guideline:

Method: other: (Pseudomanas cell multiplication inhibitiontest)

GLP compliance:

not specified

Analytical monitoring:

no

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Total exposure duration:

16 h

Details on test conditions:

Type: aquatic

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

213 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

The inhibition effect was determined by the increase of 
turbidity of  bacterial cultures at chosen logarithmic 
dilutions of the initial  concentration of the test substance

Executive summary:

The toxicity of phthalic acid to Pseusomonas putida was tested in a 16 hours test with pH adjustment to 7.4. The population growth impairment was used as endpoint. The test was performed according to the ISO 10712 method. An EC50 of 213 mg/l was observed (Sepic, 2003).

Description of key information

The effect of phthalic anhydride towards the growth of activated sludge was investigated according to ISO 8192 having an 3h-EC50 of > 1000 mg/L (Bayer AG, 1984).  The toxicity of phthalic acid to Pseusomonas putida was tested in a 16 hours test with pH adjustment to 7.4. The population growth impairment was used as endpoint and an EC50 of 213 mg/l was observed (Sepic, 2003). 

Key value for chemical safety assessment

EC50 for microorganisms:

213 mg/L

Additional information

Toxicity of phthalic anhydride to activated sludge was evaluated by Bayer AG (1984) according to the ISO 8192 yielding an EC50 value of > 1000 mg/l when exposed to phthalic anhydride for 3 hours at a pH between 7.4 and 7.6.

The toxicity of the hydrolysis product phthalic acid towards microorganisms was tested in a 16 hours test with Pseusomonas putida with pH adjustment to 7.4. The population growth impairment was used as endpoint. The test was performed according to the ISO 10712 method. An EC50 of 213 mg/l was observed (Sepic, 2003).