Reaction mass of (2R,5R)-2-isopropyl-5-methylcyclohexanone and (2S,5R)-2-isopropyl-5-methylcyclohexanone

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

Antimicrobial activities of essential oils of mint and peppermint as well as some of their main compounds

Author:

Jivoretz L, Buchbauer G, Bail S, Denkova Z, Slachev A, Stoyanova A, Schmidt E, Geissler M

Year:

2009

Bibliographic source:

Dossier CAS 14073-97-3 (Journal of Essential Oil Research 21, 363-366 )

Materials and methods

Principles of method if other than guideline:

The antimicrobial activity was studied with the agar diffusion disc method using 6 mm paper discs and quantities of 6 µL of substance. After cultivation of the bacteria and the yeast at 37 °C for 24 h, the diameter of the inhibition zone (IZ) was measured. Then, the agar serial tube dilution method was applied, yielding results as minimum inhibitory concentration (MIC) as follows: The oil and pure reference compounds were added to brine, containing 1.0% (v/v) Tween 80 at the appropriate volumes to produce final concentrations of the samples in the range of 1-1000 ppm; the Petri dishes were inoculated by pipetting 0.1 cm3 of the bacterial or yeast culture and 6 µL of the test or reference compounds on paper discs (6 mm) and then incubated at 37°C for 24 h.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(-)-menthone

IUPAC Name:

(-)-menthone

Details on test material:

- Name of test material (as cited in study report): (-)-menthone

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

other: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae, Salmonella sp., Candida albicans

Details on inoculum:

All test microorganims were obtained from the National Bank of Industrial Microorganisms and Cell Cultures, Sofia, Bulgaria

Study design

Test type:

static

Total exposure duration:

24 h

Test conditions

Test temperature:

37 °C

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24

Dose descriptor:

other: Minimum Inhibitory Concentration (MIC)

Effect conc.:

60 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Staphylococcus aureus

Effect concentrations 2

Duration:

24 h

Dose descriptor:

other: Minimum Inhibitory Concentration (MIC)

Effect conc.:

60 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Enterococcus faecalis

Effect concentrations 3

Duration:

24 h

Dose descriptor:

other: Minimum Inhibitory Concentration (MIC)

Effect conc.:

600 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Escherichia coli

Effect concentrations 4

Duration:

24 h

Dose descriptor:

other: Minimum Inhibitory Concentration (MIC)

Effect conc.:

60 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Proteus vulgaris

Effect concentrations 5

Duration:

24 h

Dose descriptor:

other: Minimum Inhibitory Concentration (MIC)

Effect conc.:

600 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Klebsiella pneumoniae

Effect concentrations 6

Duration:

24 h

Dose descriptor:

other: Minimum Inhibitory Concentration (MIC)

Effect conc.:

60 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Salmonella sp.

Effect concentrations 7

Duration:

24 h

Dose descriptor:

other: Minimum Inhibitory Concentration (MIC)

Effect conc.:

60 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Candida albicans