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Ecotoxicological information

Toxicity to terrestrial plants

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Description of key information

The effects of Amyl Salicylate on the seedling emergence and early growth of higher terrestrial plants has been determined. The lowest EC10 was 89.3 mg/kg soil dry weight.

The test soil, Speyer 2.3, had an Organic Carbon content of 0.67%.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for terrestrial plants:
89.3 mg/kg soil dw

Additional information

The seedling emergence and seedling growth test (OECD 208) covers a sensitive stage in the life-cycle of a plant and therefore data obtained from this study can be used as estimates of long-term toxicity (ECHA Chapter R.7c, section R.7.11.3.1). For long-term toxicity testing, ECHA considers six species as the minimum with two monocotyledonous species and four dicotyledonous species.


 


The test soil, Speyer 2.3, had an Organic Carbon content of 0.67%.


The effect of Amyl Salicylate was tested with six test species representing six plant families. Test species comprised two monocotyledonous, Avena sativa (oats) and Allium cepa (onion), and four dicotyledonous species, Brassica napus (oilseed rape), Cucumis sativus (cucumber), Pisum sativum (peas) and Solanum lycopersicum (formerly specified as Lycopersicon esculentum, common name = tomato).


 


The test item significantly reduced the emergence rate of S. lycopersicum (tomato) at test item concentrations of ≥667 mg/kg soil dry weight, while it did not affect survival or emergence in any other of the six plant species.


 


Shoot length was significantly reduced at test item concentrations of ≥444 mg/kg soil in S.lycopersicum and at 1000 mg/kg soil in B. napus (oilseed rape) and C. sativus (cucumber).


 


Shoot fresh weight was significantly reduced at ≥444 mg/kg soil in A. cepa (onion) and S.lycopersicum, at ≥667 mg/kg soil in C. sativus and at 1000 mg/kg soil in A. sativa (oats) and B. napus.


 


The lowest LOEC (444 mg/kg soil dry weight) and NOEC (148 mg/kg soil dry weight) were observed for shoot fresh weight in both A. cepa and S. lycopersicum. The EC10 values for this endpoint were 365 mg/kg soil dry weight (A. cepa) and 196 mg/kg soil dry weight (S.lycopersisum).


 


The lowest EC10 (89.3 mg/kg soil dry weight) was obtained for seedling emergence in S. lycopersicum. The NOEC for this endpoint was 444 mg/kg soil dry weight.


 


The preferred observational endpoint in long-term studies is the EC10 value because it is derived from the dose response curve. In contrast the NOEC depends on the experiment design (e.g. the concentrations used in the test). Therefore the lowest EC10 of 89.3 mg/kg soil dry weight has been chosen as the key value for chemical safety assessment.