2,2' -oxybisethanol;diethylene glycol

Toxicity to terrestrial arthropods

Administrative data

Endpoint:

toxicity to terrestrial arthropods: long-term

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

secondary source

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Test for Measuring Survival and Reproduction of Springtails Exposed to Contaminants in Soil. Report EPS Second Draft – August 2005. Method Development and Applications Section, Environmental Technology Centre, Environment Canada, Ottawa, Ontario.

Principles of method if other than guideline:

Study conducted according to Environment Canada test method.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 001

Sampling and analysis

Analytical monitoring:

yes

Test substrate

Vehicle:

no

Test organisms

Test organisms (species):

Folsomia candida

Animal group:

Collembola (soil-dwelling springtail)

Study design

Study type:

laboratory study

Limit test:

no

Total exposure duration:

28 d

Remarks:

Chronic Screening

Test conditions

Test temperature:

20 ± 2°C

pH (if soil or dung study):

Soil pH ranged from 6.19 to 7.64 for the beginning of all tests and from 3.92 to 7.40 for the end of all tests.

Humidity:

Soil moisture content of all treatments in all tests was independent of DEG concentrations and ranged from 59 to116% of the soil water holding capacity (WHC) for the beginning of all tests and from 44 to 91% of the soil WHC for the end of the invertebrate tests.

Photoperiod and lighting:

16 light : 8 dark; Fluorescent, 400-800 Lux

Details on test conditions:

The artificial soil was formulated in the Stantec laboratory from natural ingredients of silica sand (70%), kaolinite clay (20%), and Sphagnum sp. peat (10%) and it was buffered to a neutral pH range (6.0 to 7.5) with calcium carbonate.
Test unit (chamber): Glass 125-mL mason jar
Amount of soil: 30 g wet wt.
Number of replicate test units per treatment: 3
Number of organisms per test unit: 10

Nominal and measured concentrations:

0, 200, 400, 600, 800, 1600, 3200, 6400, 12800, 25600, 51200 mg/kg
The recovery of DEG from the beginning of the range-finding tests was good and ranged from 75 to 90%. DEG recovery on Day 0 of the northern
wheatgrass test was similar among all but the lowest (200 mg/kg) treatments and ranged from 85 to 102%. The recovery in the lowest treatment was below the detection limit. There was evidence of loss of DEG from soil over time; measurable concentrations of DEG were only detected in the highest concentration (25600 mg/kg) at Days 7 and 21 of the test. In addition, the % DEG recovery in these concentrations also decreased over time with 102, 74 and 63% recovery at Days 0, 7 and 21, respectively.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

28 d

Dose descriptor:

LC50

Effect conc.:

15 689 mg/kg soil dw

Basis for effect:

mortality

Effect concentrations 2

Duration:

28 d

Dose descriptor:

other: IC50

Effect conc.:

7 508 mg/kg soil dw

Basis for effect:

reproduction

Remarks:

number of progeny

Details on results:

Adult Folsomia candida survival was affected adversely following exposure to DEG in AS with a chronic LC50 of 15,689 mg/kg. Chronic reproduction appeared to follow a concentrationdependent pattern and the IC50 for number of progeny produced was 7508 mg/kg.
Adult survival, no. progeny produced