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EC number: 203-468-6
CAS number: 107-15-3
4-NPD: 4-NITRO-0 -PHENYLENEDIAMINE; 9-AA:
NaN3: SODIUM AZIDE; 2-AA: 2-AMINOANTHRACENE
+ indicates standard deviation of mean value
Ethylenediamine (EDA) was tested for potential mutagenic activity using the Salmonella/microsome bacterial mutagenicity assay (Ames test). Test concentrations for the Ames test were chosen from data obtained in a preliminary study with strain TA 100 performed both with and without a rat liver S9 activation system.
In tests without S9, a dose level of 1.0 mg/plate allowed only sparse growth of the background lawn and reduced the numbers of revertant colonies to less than half the control level. In contrast, tests with S9 activation allowed confluent growth of the background lawn at dose levels ranging from 0.01 to 3.0 mg/plate. A slightly higher dose of 5.0 mg/plate allowed only sparse growth of the background lawn. Based on these results, five doses ranging from 0.01 to 1.0 mg/plate were tested in the definitive test without S9 and a higher range of 0.1 to 5 mg/plate was tested in the presence of the S9 metabolic activation system. These concentrations were tested with five different strains of Salmonella typhimurium (TA98, TA100, TA1535, TA1537, and TA1538) using triplicate cultures at each dose level for each strain.
In the definitive mutagenicity test performed without S9, no evidence of positive or dose related mutagenic activity was observed with any of the five strains. In tests performed in the presence of an Aroclor 1254 induced rat-liver S9 metabolic activation system, only strain TA100 showed a slight increase in numbers of revertant colonies (the magnitude of the response at the highest concentration levels ranged from 1.7 to 1.9 times the concurrent controls) but this was less than a 2-fold increase. In the first test with S9, TA1535 showed a slight increase; however, this response was within the historical control range of variation for this strain and was not reproduced in two additional experiments. Overall, because of a less than 2-fold increase obtained with TA100 tested with S9, and no increases were seen using strains TA 98, TA 1535, 1537 and 1538 with and without metabolic activation, EDA was considered not to be mutagenic in this in vitro bacterial assay.
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