Ethylenediamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

according to guideline

Guideline:

other: Japanese Industrial Standards Committe 1971

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on test solutions:

The test substance was diluted with water and neutralised with HCl.

Test organisms (species):

Oryzias latipes

Details on test organisms:

Fish of the same age about 2 cm in length and 0.2 g in weight were used

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

No data

Test temperature:

No data

pH:

No data

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

No data

Details on test conditions:

No data

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

1 000 mg/L

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Conclusions:

The 48-h LC50 for Oryzia latipes was 1000 mg/L.

Executive summary:

A study was conducted to determine the acute toxicity potential of the test substance to Oryzias latipes (Japanese rice fish), according to Japanese Industrial Standards Committee 1971. Very limited details on the study are available to allow for evaluation. The fish were exposed to the test substance for 48 h. Under the study conditions, the 48 h LC50 was determined to be 1000 mg/L (Tonogai, 1982).

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

not specified

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on test solutions:

Test medium Dutch Standard Water.

Test organisms (species):

Poecilia reticulata

Details on test organisms:

No data

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

96 h

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 544.7 mg/L

Basis for effect:

mortality (fish)

Conclusions:

A 96-h LC50 value of 1544 mg/L was reported for freshwater fish Poecilia reticulata.

Executive summary:

A semi-static study was conducted to determine the acute toxicity of the test substance to Poecilia reticulata, according to EU Method C.1 Guideline. Only limited information on the study conduct, method and results are available to allow evaluation. Under the study conditions, the 96 h LC50 (median lethal concentration) was determined to be 1544.7 mg/L (van Wijk, 1994).

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Note, no analytical monitoring of test substance concentrations were performed.

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Remarks:

Ethylene diamine has a high water solubility, is chemically stable and does not sorb significantly to glassware which indicates that the organisms were exposed to the nominal test concentrations.

Details on test solutions:

A stock solution of 26 mg/l was prepared in de-mineralized water. pH was adjusted with HCl to a pH between 7.0 and 7.3 both in stock solution and in dilution water. Dilution water was a natural water from the aquarium where the fish were kept.

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Source: Produced at the laboratory
- Length at study initiation : 2 cm

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

63.7 mgCa/l, 31.8 mg MG/l, 16.2 °dH

Test temperature:

22.8- 23.6°C

pH:

7.0 - 8.0

Dissolved oxygen:

7.2 - 8.5 mg/l

Nominal and measured concentrations:

0 - 180 - 320 - 560 - 1000 - 1800 mg/l nominal

Details on test conditions:

TEST SYSTEM
- Test vessel:2 l
- Type: open
- Material, size, headspace, fill volume: Glass aquaria with 1 l medium
- Aeration: No
- Renewal rate of test solution: Renewed after 48 h
- No. of organisms per vessel: 10
- No. of replicates: 1
- No. of vessels per control (replicates): 6 vessels containing 10 fish

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Aquarium water
- Culture medium different from test medium: No

OTHER TEST CONDITIONS
- Adjustment of pH: Yes in stock solutions
- Photoperiod: 12 h

EFFECT PARAMETERS MEASURED: Mortality

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0- 10- 100 - 1000 mg/l.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

640 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Remarks on result:

other: 470-870

Validity criteria fulfilled:

yes

Conclusions:

Ethylenediamine is found not harmful to fish in an acute toxicity test. The 96-h LC50 is 640 mg/l.

Executive summary:

A semi-static study was conducted to determine the acute toxicity of the test substance to Poecilia reticulata, according to EU Method C.1, in compliance with GLP. No analytical monitoring of the test substance concentration was peformed in this study. The study was performed in 6 vessels, each containing 10 fish. The test concentrations were presented as nominal concentrations i.e., 0, 180, 320, 560, 1000 and 1800 mg/L during 96h exposure. No significant mortality was observed up to 320 mg/L. Validity criteria of the study was reported to be fulfilled. Under the study conditions, the 96 h LC50 was determined to be 640 mg/L, with 95% confidence limits of 470 and 870 mg/L (Balk, 1989).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

640 mg/L

Additional information

A semi-static study was conducted to determine the acute toxicity of the test substance to Poecilia reticulata according to EU Method C.1, in compliance with GLP. No analytical monitoring of the test substance concentration was performed. The study was performed in 6 vessels, each containing 10 fish. The test concentrations were presented as nominal concentrations i.e., 0, 180, 320, 560, 1000 and 1800 mg/L during 96 h exposure. No significant mortality was observed up to 320 mg/L. Validity criteria of the study was reported to be fulfilled. Under the study conditions, the 96 h LC50 was determined to be 640 mg/L, with 95% confidence limits of 470 and 870 mg/L (Balk, 1989).

A semi-static study was conducted to determine the acute toxicity of the test substance to Poecilia reticulata according to EU Method C.1 Guideline. Only limited information on the study conduct, method and results are available to allow evaluation. The fish were exposed to the test substance for 96 h. Under the study conditions, the 96 h LC50 (median lethal concentration) was determined to be 1544.7 mg/L (van Wijk, 1994).

A study was conducted to determine the acute toxicity potential of the test substance to Oryzias latipes (Japanese rice fish) according to Japanese Industrial Standards Committee 1971. Only limited details on the study were available to allow for evaluation. The fish were exposed to the test substance for 48 h. Under the study conditions, the 48 h LC50 was determined to be 1000 mg/L (Tonogai, 1982).