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Linear alkylbenzenes, at concentrations up to and exceeding the water solubility limit, had no short-term effects on all three trophic levels tested (fish, invertebrates and algae). Following ECHA Guidance on the Application of the CLP Criteria (2012), which recommends testing of complex substances having a range of water solubilities (such as LAB) using the Water Accommodated Fraction (WAF) method, a GLP-compliant OECD 211 guideline Daphnia magna study was conducted using the WAF dosing method (Minderhout et al. 2013). In this study there were no biological or reproductive effects noted. The 21-d NOEL is > 10 mg/L (WAF), the highest dose tested. Based on these results, LAB is not classified for chronic aquatic toxicity.