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Diss Factsheets

Toxicological information

Additional toxicological data

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Administrative data

Endpoint:
additional toxicological information
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Study period:
published in 1974, no experimental dates provided
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Research paper study well documented meeting generally accepted scientific principles.

Data source

Reference
Reference Type:
publication
Title:
Food iron absorption in man. Applications of the two-pool extrinsic tag method to measure heme and nonheme iron absorption from the whole diet.
Author:
Bjorn-Rassmussen E, Hallberg L, Isaksson B et al.
Year:
1974
Bibliographic source:
J. Clin. Invest. 53: 247-255.

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
The method is based on the concept that food iron is absorbed from two pools the haem iron and the non-haem iron pool which can be labelled by the use of two  
radio iron isotopes given as haemoglobin (59 Fe-labelled rabbit haemoglobin in the diet) and as an iron salt (55 and 59 Fe labelled ferric chloride). 
The men were fed the radiolabelled iron materials in the diet supplied as breakfast, lunch and dinner.
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Iron sulphate
EC Number:
231-753-5
EC Name:
Iron sulphate
Cas Number:
7720-78-7
IUPAC Name:
iron(2+) sulfate
Test material form:
other: haem and non-haem dietary iron
Details on test material:
Male subjects were fed the radiolabelled iron materials in the diet supplied as breakfast, lunch and dinner

Results and discussion

Any other information on results incl. tables

The main findings from this study indicate that there was a greater absorption of haem iron (35%) than non-haem iron (5%) in these human 

volunteers. There was a significant increase in iron absorption when the level of iron fortification of the meals was increased. The test method was 

considered to acceptable for the measurement of total iron absorption from a mixed diet.

Applicant's summary and conclusion

Conclusions:
The main findings from this study indicate that there was a greater absorption of haem iron (35%) than non-haem iron (5%) in these human 
volunteers. There was a significant increase in iron absorption when the level of iron fortification of the meals was increased. 
Executive summary:

This study was undertaken to test the accuracy of the two pool intrinsic tag method to measure haem and non-haem iron in the whole diet.

32 young male volunteers were used to assess the accuracy of an isotope test method to measure iron absorption from the whole diet. The method is based on the concept that food iron is absorbed from two pools the haem iron and the non-haem iron pool which can be labelled by the use of two  

radio iron isotopes given as haemoglobin (59 Fe-labelled rabbit haemoglobin in the diet) and as an iron salt (55 and 59 Fe labelled ferric chloride). 

The men were fed the radiolabelled iron materials in the diet supplied as breakfast, lunch and dinner.

The main findings from this study indicate that there was a greater absorption of haem iron (35%) than non-haem iron (5%) in these human 

volunteers. There was a significant increase in iron absorption when the level of iron fortification of the meals was increased.