2-methyl-1-heptanol

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was well documented and meets generally accepted scientific principles, but was not conducted in compliance with GLP.

Data source

Reference

Reference Type:

publication

Title:

Comparison of the toxicity thresholds of water pollutants to bacteria, algae, and protozoa in the cell multiplication inhibition test.

Author:

Bringmann G, Kuhn R

Year:

1980

Bibliographic source:

Water Research 14(3): 231-241.

Materials and methods

Test guideline

Qualifier:

no guideline followed

GLP compliance:

not specified

Test material

Reference

Name:

Unnamed

Type:

Constituent

Sampling and analysis

Analytical monitoring:

not specified

Test organisms

Test organisms (species):

Entosiphon sulcatum

Study design

Test type:

other: Cell multiplication inhibition test

Water media type:

freshwater

Total exposure duration:

72 h

Remarks on exposure duration:

Incubation period

Test conditions

Test temperature:

25 degC

Details on test conditions:

TEST SYSTEM
- Test vessel: 300 ml Erlenmeyer flasks

- Test vessel content: 8ml culture liquid. 8ml stock solution I, 2ml united preliminary protozoan cultures (approx. impulses 15000/ml)I, and 10ml each of bacterial suspension (or 10ml each of saline for the dilution series not inoculated) and 2ml of suspension of inactivated bacteria from a preliminary bacterial culture having a known extinction value.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Double distilled water

OTHER TEST CONDITIONS
- Adjustment of pH: Pollutant solution adjusted to pH6.9 using the minimum volume of hydrochloric acid or caustic potash.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- After incubating, add 10% of a 1.1%NaNO3 solution in double distilled water filtered through a membrane filter (pore size 0.2um) to each falsk and determine the number of protozoa by means of a cell counter.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: Four parallel dilution series, 1:2 to 1:2E+14 v/v. One series inoculated, one not.

Reference substance (positive control):

not required

Remarks:

not applicable

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

other: TT or EC3

Effect conc.:

44 mg/L

Basis for effect:

other: cell multiplication inhibition

Applicant's summary and conclusion

Conclusions:

A toxicity threshold (TT) value for bacteria (Entosiphon sulcatum) of 44 mg/l was determined in a reliable study conducted according to generally accepted scientific principles.