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Diss Factsheets

Administrative data

activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
31st Oct 2007 - 15th Nov 2007
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study performed in accordance with GLP.

Data source

Reference Type:
study report

Materials and methods

Test guideline
according to guideline
other: OECD test guideline 209
GLP compliance:

Test material

Constituent 1
Test material form:
other: Liquid

Sampling and analysis

Analytical monitoring:
not required

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Collected on each day of the test from Cambridge Sewage Treatment Works, Cowley Road, Cambridge.

Study design

Test type:
Total exposure duration:
3 h

Test conditions

Test temperature:
21 ± 2 °C
Not specified
Dissolved oxygen:
Monitored using an oxygen electrode - abiotic control (1000mg/) : 8.51 mg/L dissolved oxygen at T0.
Nominal and measured concentrations:
Nominal concentrations : 62.5, 125, 250, 500, 1000, 2000 mg/L
Reference substance (positive control):
3, 5-Dichlorophenol (3,5-DCP)

Results and discussion

Effect concentrations
3 h
Dose descriptor:
Effect conc.:
593 mg/L
Nominal / measured:
Basis for effect:
inhibition of total respiration
respiration rate
Results with reference substance (positive control):
- Results with reference substance valid? Yes

Applicant's summary and conclusion

Validity criteria fulfilled:
The IC50 of Inhibitor AHM P500 to the respiration rate of activated sludge was estimated as 593 mg/L (95% confidence limits of 343 to1288 mg/L. The validity controls were considered acceptable.
Executive summary:

A GLP study was performed in accordance with OECD 209 in order to assess the effect of Inhibitor AHM P500 to the respiration rate of activated sludge.

Based on the results of the preliminary test, nominal concentrations of 62.5 – 2000mg/L were used in the test. The test was run at 21±2°C. The dissolved oxygen was determined using an oxygen meter for a period of 10 minutes after an incubation period of 3hours from inoculation. A positive control (3,5-DCP) was included in the test.

Based on the results, the IC50 of Inhibitor AHM P500 to the respiration rate of activated sludge was estimated to be 593 mg/L (95% confidence limits of 343 to1288 mg/L). The validity controls were considered acceptable.