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Toxicity to microorganisms

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Description of key information

The 3-h EC50 value is >1000 mg/L in microorganisms	

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information

The inhibitory effect of the testsubstance on the respiration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test according to the EU Commission Directive 88/302/EEC, Part C.11, and the OECD Guideline for Testing of Chemicals, No. 209.

In accordance with the EU Commission Directive 92/69/EEC (C.1, C.2, and C.3), a limit test with a nominal test concentration of 1000 mg/L was performed to demonstrate that the test item has no toxic effect on activated sludge microorganisms up to and including this concentration. In addition, two controls and three different concentrations of the reference item 3,5-dichlorophenol (5, 16, and 50 mg/L) were tested in parallel. The results of these treatments confirmed suitability of the activated sludge and the method used.

The test item had no inhibitory effect on the respiration rate of activated sludge after the incubation period of three hours at the test item concentration of 1000 mg/L. Thus, the 3-hour NOEC (EC15) of the test ilem to activated sludge microorganisms was at least 1000 mg/L. This value might even be higher but concentrations above 1000 mg/L were not tested. The 3-hour EC20, EC50, and EC80 could not be calculated, but were clearly higher than 1000 mg/L.

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