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EC number: 483-940-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From January 11, 2010 to March 25, 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- - Source: In-house laboratory culture
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- 0, 24, 48 and 72 hours
- Nominal and measured concentrations:
- 0.1, 1.0, 10 and 100 mg/l
- Details on test conditions:
- - Test vessel: 250ml tissue culture flask
- Test volume: 30 mL
- No. of vessels per concentration (replicates): 6 replicates for the highest test concentration and 3 replicates for other test concentration
- No. of vessels per control (replicates): 6
- Light intensity: 114-127 μE.m-2.s-1 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- for growth rate reduction
- Effect conc.:
- > 92 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- for yield inhibition
- Effect conc.:
- >= 8.4 - <= 92 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Results with reference substance (positive control):
- The EC50 for growth rate reduction (ERC50: 0-72h) was 1.2 mg/l and the EC50 for yield inhibition (EYC50: 0-72h) was 0.65 mg/l.
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to OECD 201 test method, the 72h-ERC50 and the EYC50 of Everzol Red CDN Crude was >92 mg/L and 8.4-92 mg/L respectively.
- Executive summary:
This test using the procedures outlined in the NOTOX Study Plan for 492365 and OECD 201 (OECD, 2006). The results of this OECD 201 test for Everzol Red CDN Crude show that test validity criteria was met. In the alga growth inhibition test, the nominal test article concentrations applied were 0.1, 1.0, 10 and 100 mg/L. The average exposure concentration were calculated: 0.083, 0.84, 8.4 and 92 mg/L. Under the test conditions, the EC50 for growth rate reduction and for yield inhibition of Everzol Red CDN Crude was >92 mg/L and 8.4-92 mg/L respectively.
Reference
Table 1.Mean cell densities (× 104 cells/ml)
Average conc. Test substance (mg/l) |
Exposure time (hours) |
|||
0 |
24 |
48 |
72 |
|
Control |
1.0 |
10.0 |
95.9 |
488.8 |
0.083 |
1.0 |
8.2 |
78.6 |
435.2 |
0.84 |
1.0 |
8.4 |
82.1 |
448.3 |
8.4 |
1.0 |
8.7 |
72.5 |
339.3 |
92 |
1.0 |
8.9 |
58.0 |
137.7 |
Table 2. Percentage reduction of growth rate (total test period) and percentage inhibition of yield
Average conc. Test substance (mg/l) |
Mean growth rate |
Yield (0-72 h) |
||
μ (0-72 h) |
Reduction (%) |
×104cells/ml |
Inhibition (%) |
|
Control |
0.08599 |
|
487.80 |
|
0.083 |
0.08438 |
1.9 |
434.20 |
11.0 |
0.84 |
0.08479 |
1.4 |
447.32 |
8.3 |
8.4 |
0.08093 |
5.9 |
338.34 |
30.6 |
92 |
0.06837 |
20.5 |
136.70 |
72.0 |
Table 3. Effect parameters
Parameter |
Concentration Everzol Red CDN Crude(mg/l) |
72h-ERC50 |
> 92 |
72h-EY50 |
8.4-92 |
Description of key information
Respectively, the 72h-ERC50 and the 72-EYC50 of Everzol Red CDN Crude was >92 mg/L and 8.4-92 mg/L for Pseudokirchneriella subcapitata (OECD TG201).
Key value for chemical safety assessment
Additional information
The cell densitiesin the control have increased by an average factor of at least 16 within 2 days. The mean coefficient of variation for section-by-section specific growth rates in the control group were not exceed 35%. The coefficient of variation of average specific growth rates during the whole test period in replicate control groups were not exceed 7%. This test validity criteria was met. Under the test conditions, the EC50 for growth rate reduction and for yield inhibition of Everzol Red CDN Crude was >92 mg/L and 8.4-92 mg/L respectively.
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