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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From January 11, 2010 to March 25, 2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: In-house laboratory culture
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
24 and 48 hours.
Nominal and measured concentrations:
0.1, 1.0, 10 and 100 mg/l
Details on test conditions:
- Test volume: 80 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 replicates for the highest test concentration and 2 replicates for other test concentration
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr photoperiod daily
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
The 48h-EC50 was 0.38 mg/l.

Table 1. Acute immobilization of daphnids after 24 and 48 hours

Concentration Everzol Red CDN Crude (mg/l)

Vessel number

Number Daphnia exposed

Response at 24 h

Response at 48 h

number

Total

%

number

Total

%

control

A

5

0 (4)

0

0 (3)

10

B

5

0 (1)

0 (1)

C

5

0 (5)

1 (2)

D

5

0 (4)

1 (5)

0.10

A

5

0 (5)

0

0 (3)

0

B

5

0 (2)

0 (3)

1.0

A

5

0 (1)

0

0 (1)

0

B

5

0 (2)

0

10

A

5

0 (3)

0

0 (2)

0

B

5

0 (3)

0 (1)

100

A

5

0

0

0

0

B

5

0

0

C

5

0

0

D

5

0

0

Between brackets: number of daphnids trapped at the surface. There daphnids were reimmersed into the respective solution before recording of immobility.

Table 2. Effect parameters

Parameter

Concentration Everzol Red CDN Crude (mg/l)

NOEC

100

24 & 48h-EC50

>100

Table 3. pH and oxygen concentrations

ConcentrationEverzol Red CDN Crude(mg/l)

Start (t=0 h)

t=24 h old

t=24 h fresh

End (t=48 h)

pH

O2

pH

O2

pH

O2

pH

O2

control

7.6

9.2

7.4

8.8

7.4

9.0

7.7

8.9

100

7.6

9.2

7.7

8.7

7.4

9.0

7.5

8.9

Validity criteria fulfilled:
yes
Conclusions:
According to OECD 202 test method, the 48-hr EC50 of Everzol Red CDN Crude was >100 mg/L.
Executive summary:

This test using the procedures outlined in the NOTOX Study Plan for 492366 and OECD 202 (OECD, 2004). The results of this OECD 202 test for Everzol Red CDN Crude show that all validity criteria were met. Under the conditions of this test, the 48-hr EC50 of Everzol Red CDN Crude was >100 mg/L.

Description of key information

The 48-hr EC50 of Everzol Red CDN Crude was >100 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

Not more than 10 % of the Daphnia were immobilised in the control and the dissolved oxygen concentration at the end of the test was ≥3 mg O2/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline for limit test. Under the conditions of this test, the 48h-EC50of Everzol Red CDN Crude was greater than 100.00 mg/L.