Everzol Red CDN Crude

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From January 11, 2010 to March 25, 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: In-house laboratory culture

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hours.

Nominal and measured concentrations:

0.1, 1.0, 10 and 100 mg/l

Details on test conditions:

- Test volume: 80 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 replicates for the highest test concentration and 2 replicates for other test concentration
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr photoperiod daily

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The 48h-EC50 was 0.38 mg/l.

Table 1. Acute immobilization of daphnids after 24 and 48 hours

Concentration Everzol Red CDN Crude (mg/l)	Vessel number	Number Daphnia exposed	Response at 24 h		Response at 48 h
			number	Total %	number	Total %
control	A	5	0 (4)	0	0 (3)	10
	B	5	0 (1)		0 (1)
	C	5	0 (5)		1 (2)
	D	5	0 (4)		1 (5)
0.10	A	5	0 (5)	0	0 (3)	0
	B	5	0 (2)		0 (3)
1.0	A	5	0 (1)	0	0 (1)	0
	B	5	0 (2)		0
10	A	5	0 (3)	0	0 (2)	0
	B	5	0 (3)		0 (1)
100	A	5	0	0	0	0
	B	5	0		0
	C	5	0		0
	D	5	0		0

Between brackets: number of daphnids trapped at the surface. There daphnids were reimmersed into the respective solution before recording of immobility.

Table 2. Effect parameters

Parameter	Concentration Everzol Red CDN Crude (mg/l)
NOEC	100
24 & 48h-EC50	>100

Table 3. pH and oxygen concentrations

ConcentrationEverzol Red CDN Crude(mg/l)	Start (t=0 h)		t=24 h old		t=24 h fresh		End (t=48 h)
	pH	O2	pH	O2	pH	O2	pH	O2
control	7.6	9.2	7.4	8.8	7.4	9.0	7.7	8.9
100	7.6	9.2	7.7	8.7	7.4	9.0	7.5	8.9

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 202 test method, the 48-hr EC50 of Everzol Red CDN Crude was >100 mg/L.

Executive summary:

This test using the procedures outlined in the NOTOX Study Plan for 492366 and OECD 202 (OECD, 2004). The results of this OECD 202 test for Everzol Red CDN Crude show that all validity criteria were met. Under the conditions of this test, the 48-hr EC₅₀ of Everzol Red CDN Crude was >100 mg/L.

Description of key information

The 48-hr EC₅₀ of Everzol Red CDN Crude was >100 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Not more than 10 % of the Daphnia were immobilised in the control and the dissolved oxygen concentration at the end of the test was ≥3 mg O₂/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline for limit test. Under the conditions of this test, the 48h-EC₅₀of Everzol Red CDN Crude was greater than 100.00 mg/L.