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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From January 23, 2008 to April 15, 2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Qualifier:
according to
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
Version / remarks:
Modified Sturm Test
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: Waterschap de Maaskant, ‘s-Hertogenbosch, The Netherlands
Duration of test (contact time):
28 d
Initial conc.:
12 mg/L
Based on:
TOC
Reference substance:
acetic acid, sodium salt
Remarks:
Sodium acetate
Parameter:
% degradation (CO2 evolution)
Value:
24
Sampling time:
28 d
Remarks on result:
other: The percentage biodegradation of Everzol Red CDN Crude was not achieve 60 % with following 10 days.
Results with reference substance:
At Day 14 during the study, the biodegradation was 72 %.

Positive control: reference substance and inoculum.

Toxicity control: test substance, reference substance and inoculum.

 

The total CO2 release in the blank at the end of the test was 22.8 mg/l.

 

Table 1. The percentage biodegradation of Positive control, Toxicity control and Test substance

Day

Biodegradation (%)

Positive control

Toxicity control

Test substance

Bottle A

Bottle B

Mean A and B

ΔA and B1

2

16

9

3

3

3

1

5

41

24

12

12

12

0

7

55

29

12

15

14

2

9

62

31

14

16

15

2

14

72

37

17

18

17

2

19

78

40

19

20

19

1

23

80

42

20

21

20

1

27

81

43

22

22

22

0

29

82

44

22

24

23

1

29

82

44

22

25

23

2

29

82

44

22

26

24

3

1Absolute difference in biodegradation between bottles A and B

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
According to OECD 301B test method and EC Method C.4-C, Everzol Red CDN Crude was not readily biodegradable.
Executive summary:

This test using the procedures outlined in the NOTOX Study Plan for 487481, OECD 301B (OECD, 1992) and EC Method C.4-C (1992). The results of this test for Everzol Red CDN Crude show that all validity criteria were met. Everzol Red CDN Crude was not be considered readily biodegradable under the test conditions.

Description of key information

Not readily biodegradable (OECD TG 301B and EC Method C.4-C).

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable, not fulfilling specific criteria

Additional information

The total CO2 release in the blank at the end of the test did not exceed 40 mg/l. The positive control was degraded by 72 % within 14 days and the difference of duplicate values for %-degradation of Everzol Red CDN Crude was always less than 20. All test validity criteria were met. Toxicity control was 37% degradation occurred within 14 days. Therefore, the test substance was assumed not to inhibit microbial activity. The percentage biodegradation of Everzol Red CDN Crude was not achieve 60 % with following 10 days. Therefore, Everzol Red CDN Crude was not the readily biodegradable.