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EC number: 265-158-7 | CAS number: 64742-55-8 A complex combination of hydrocarbons obtained by treating a petroleum fraction with hydrogen in the presence of a catalyst. It consists of hydrocarbons having carbon numbers predominantly in the range of C15 through C30 and produces a finished oil with a viscosity of less than 100 SUS at 100°F (19cSt at 40°C). It contains a relatively large proportion of saturated hydrocarbons.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- No data reported
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study is classified as reliable without restriction because it was carried out in a method similar to OECD TG 471 with modifications.
- Justification for type of information:
- The standard OECD 471 test is not suitable to test petroleum UVCBs, because it has a tendency to produce false negatives for these substances. Therefore, the petroleum industry has developed a Modified Ames assay, optimized to accurately identify positive results for this endpoint. This deviation from the prescribed testing procedure requires some further explanation which is given in the attached document. The document gives a brief history of the development of the Modified Ames test and outlines Concawe’s proposed work (as part of a wider testing strategy, see Annex 13) to further support the use of this test for PS.
Data source
Reference
- Reference Type:
- publication
- Title:
- Estimation of the dermal carcinogenic activity of petroleum fractions using a modified Ames assay
- Author:
- Blackburn GR, Deitch RA, Schreiner CA, Mehlman MA, and Mackerer CR
- Year:
- 1 984
- Bibliographic source:
- Cell Biol Toxicol, Vol 1(1), pp 67-80
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- with modifications
- Principles of method if other than guideline:
- Method: other: procedure described in paper by Blackburn et al. The light paraffinic distillate was extracted with dimethylsulphoxide by performing five successive one-to-one extractions and pooling extracts. The pooled extract sample was stored at 4 degrees celsius in an amber bottle until assayed.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 64741-50-0 (> 3% IP 346)
- IUPAC Name:
- 64741-50-0 (> 3% IP 346)
- Reference substance name:
- Light paraffinic distillate
- IUPAC Name:
- Light paraffinic distillate
- Test material form:
- other: Oily liquid
- Details on test material:
- Test substance:
Sample number 3 CAS 64741-50-0
Constituent 1
Constituent 2
Method
- Target gene:
- Not applicable
Species / strain
- Species / strain / cell type:
- other: Modified Ames Test using Salmonella typhimurium strain TA 98 and Aroclor 1254-induced rat or hamster liver S-9
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with
- Metabolic activation system:
- Aroclor 1254-induced rat or hamster liver S9
- Test concentrations with justification for top dose:
- range of doses that provided an initial linear dose response; specific doses not stated.
- Vehicle / solvent:
- No data reported.
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- benzo(a)pyrene at 5 ug/plate and 2-aminoanthracene at 2 ug/plate
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- The light paraffinic distillate was extracted with dimethylsulphoxide by performing five successive one-to-one extractions and pooling extracts. The pooled extract sample was stored at 4 degrees celsius in an amber bottle until assayed.
- Evaluation criteria:
- A tangent to the dose-response curve at zero dose was constructed to provide the Mutagenicity Index.
- Statistics:
- A tangent to the dose-response curve at zero dose was constructed to provide the Mutagenicity Index.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Remarks:
- Not applicable, modified Ames protocol
- Species / strain:
- S. typhimurium TA 1537
- Remarks:
- Not applicable, modified Ames protocol
- Species / strain:
- S. typhimurium TA 100
- Remarks:
- Not applicable, modified Ames protocol
- Species / strain:
- S. typhimurium TA 102
- Remarks:
- Not applicable, modified Ames protocol
- Remarks on result:
- other: other: Modified Ames Test using Salmonella typhimurium strain TA 98 and Aroclor 1254-induced hamster liver S-9
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The test substance was not mutagenic in standard assay, but extraction with DMSO produced a mutagenic response. The mutagenicity index was 17. This compares with values of 0 for a solvent refined oil and 40 for a cracked distillate.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive with metabolic activation
DMSO-extracted light paraffinic distillate was classified as mutagenic and produced a mutagenicity index of 17. Assay sensitivity (the number of TA98 revertants per plate) improved with 8-fold increase of S9. - Executive summary:
In a modified in vitro mutagenicity assay, S. typhimurium strain TA98 was exposed to a DMSO-extracted light paraffinic distillate at a concentration of 50 microlitres per plate with metabolic activation (Aroclor 1254 -induced rat or hamster liver S9) using the standard pre-incubation method. The test substance was considered mutagenic and produced a mutagenicity index of 17. This study received a Klimisch score of 1 and is classified as reliable without restriction because it was carried out in a method similar to OECD TG 471 with modifications.
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