Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
not required

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
source: Shanghai Longhua Sewage Plants
activity and sensitivity of the microbial innoculum was tested with copper sulfate

Study design

Limit test:
yes
Total exposure duration:
30 min

Test conditions

Test temperature:
20°C
pH:
6-8
Nominal and measured concentrations:
nonimal concentration 100 mg/l
Details on test conditions:
two replicates
Contact between innoculum and the test substance: 30 min
Respiration rate measured over a period of 10 min
Reference substance (positive control):
yes
Remarks:
copper sulfate

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC50 (30 min) = 39.8 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Test substance has no inhibitory effect on activated sludge respiration under these test conditions.