Pentacarbonyliron

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions. No analytical dose verification was performed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38 412 L15

Principles of method if other than guideline:

Comparable to OECD 203.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Pentacarbonyliron

EC Number:

236-670-8

EC Name:

Pentacarbonyliron

Cas Number:

13463-40-6

Molecular formula:

C5FeO5

IUPAC Name:

pentacarbonyliron

Details on test material:

- Name of test material (as cited in study report): Pentacarbonyliron
- Batch No.: 2.6.87
- Purity: >99.5%
- Solubility in water: about 70 mg/l

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenweststedt, Germany
- Length at study initiation (mean, range): 5.3 cm (range 4.7-5.7 cm)
- Weight at study initiation (mean and range, SD): 3.7 g (range 2.1-4.9 g)
- Feeding during test: no food


ACCLIMATION
- Acclimation period: 4 weeks
- Acclimation conditions (same as test or not): Fish were kept in a flow through tank in tap water cleaned by active carbon and aerated.
- Photoperiod: 16h:8h (L/D)
- Total hardness: about 2.5 mmol/l
- Acid capacity: about 5.5 mmol/l
- Oxygen content: > 60% of maximum saturation
- pH: about 8.0
- Type and amount of food: growing feed F/B 50 SSNIFF Spezialdiaeten GmbH D-4770 Soest, Frg Ad libitum
- Feeding frequency:
- Health during acclimation (any mortality observed): 0% mortality
- Medical treatment: twice with 0.05 mg/l Malchite green chloride once with 10 mg/l Tetracycline hydrochloride.
- Acclimation to test water and test temperature: 3 days
- Withdrawal of food: 1 day before and during exposure.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

20 ±1 °C

pH:

7.8 - 8.1

Dissolved oxygen:

8.7 - 7.8 mg/L

Nominal and measured concentrations:

Nominal test concentrations: 3.16-2150 mg/l (based on the results of a range-finding test).

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: All glass aquaria (30 x 22 x 24 cm); 10 L test solution.
- Aeration: slight
- No. of organisms per vessel: 10
- Biomass loading rate (g fish/l test water): 3.7


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982 prepared from fully demineralised tap water (conductivity: max 10 micro MHO) by adding: 294.0 mg/l CaCl2 * 2 H2O 123.3 mg/l MgSO4 + 7 H2O 64.8 mg/l NaHCO3 5.8 mg/l KCl
- Ca/mg ratio: 4 : 1
- Na/K ratio: 10 : 1


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime


TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: LC50 after 96 h between 100 and 1000 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

991.23 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % C.I. = 621-2019 mg/L

Any other information on results incl. tables

Sublethal observations / clinical signs:

Nominal Number Cumulative mortality
conc. of fish
(mg/l) 1h/4h/24h/48h/72h/96h
-----------------------------------------------------------
3.16 10 0 0 0 0 0 0
10.00 10 0 0 0 0 0 0
31.60 10 0 0 0 0 0 0
100.0 10 0 0 0 0 0 0
215.0 10 0 0 0 1 1 1
464.0 10 0 0 0 2 2 3
1000.0 10 0 0 1 2 3 4
2150.0 10 0 2 5 6 6 8
0.0 10 0 0 0 0 0 0
-----------------------------------------------------------

Testing for homogenity: yes, homogene
LC50 (96h): 991.23 mg/l (CI 620.92-2018.98)
LC05 (96h): 168.04 mg/l (CI 30.36-315.25)

Symptoms of intoxication:

Nominal Symptoms
conc.
(mg/l) 1h/4h/24h/48h/72h/96h
-----------------------------------------------------------
3.16 10 - - - - - -
10.00 10 - - - - - -
31.60 10 - - - - - -
100.0 10 - - - - - -
215.0 10 - - T - - -
464.0 10 - - - - - A
1000.0 10 - - - - - -
2150.0 10 T T - - - -
0.0 10 - - - - - -
-----------------------------------------------------------
Explanation of symptoms:
A=Apathy
T=Tumbling

All results refer to nominal test concentrations of pentacarbonyliron.

No analytical dose-verification of the test item was carried out.