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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian germ cell study: cytogenicity / chromosome aberration
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: According to the OECD SIDS document for sodium hydroxide (March 2002), a Klimisch 3 reliability score was assigned since an unsuitable test system is used.

Data source

Reference
Reference Type:
publication
Title:
Spermatocyte chromosome aberrations in two species of grasshoppers at two different ionic activities
Author:
Manna et al.
Year:
1966
Bibliographic source:
Nucleus, 9, 119-131

Materials and methods

Principles of method if other than guideline:
Species: grasshopper (Spathosternum prasiniferum) the grasshoppers were injected abdominally with 0.02 ml of a pH 9 NaOH solution and the testes were fixed after intervalls of 4, 18, and 24 h; no validated test
GLP compliance:
no
Type of assay:
chromosome aberration assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium hydroxide
EC Number:
215-185-5
EC Name:
Sodium hydroxide
Cas Number:
1310-73-2
Molecular formula:
HNaO
IUPAC Name:
sodium hydroxide
Details on test material:
no data

Test animals

Species:
other: grasshopper
Strain:
other: Spathosternum
Sex:
not specified

Administration / exposure

Route of administration:
intraperitoneal

Results and discussion

Test results
Sex:
not specified
Genotoxicity:
other: see additional information on results
Toxicity:
not specified
Vehicle controls validity:
not specified
Negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
Marked changes were observed in the spermatocyte chromosomes of the 24-h specimens; the frequency of chromatid and chromosome type breaks was 3.2% (18/564 cells examined); other abnormalities included multipolar spindels, asynchronous separation of chromosomes, distribution of chromosomes in small groups, extreme stickiness and clumping of chromosomes, and sticky bridges.

Applicant's summary and conclusion