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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Older published study containing limited information

Data source

Reference
Reference Type:
publication
Title:
The single dose toxicity of some glycols and derivates
Author:
Smyth, H.F. Seaton, J. Fischer, L.
Year:
1941
Bibliographic source:
Journal of Industrial Hygiene and Toxicology, 23(6): 259-268

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Principles of method if other than guideline:
Acute toxicity following single dose administration
GLP compliance:
no
Remarks:
: study pre-dates GLP
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Ammonia, anhydrous
EC Number:
231-635-3
EC Name:
Ammonia, anhydrous
Cas Number:
7664-41-7
Molecular formula:
H3N
IUPAC Name:
ammonia
Details on test material:
The test substance was aqueous ammonia; anhydrous ammonia in solution.

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
Male Wistar rats, weighing 50-120g at study initiation

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
Single dose by gavage
Doses:
No information
No. of animals per sex per dose:
10 male rats per dose
Control animals:
not specified
Details on study design:
Observations were made for 14 days after dosing
Statistics:
Probit method

Results and discussion

Preliminary study:
Not relevant
Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
350 mg/kg bw
Remarks on result:
other: Probit analysis
Mortality:
No further details are reported
Clinical signs:
other: No information
Gross pathology:
No information
Other findings:
No information

Any other information on results incl. tables

The LD50 of ammonium hydroxide in water was 350 mg/kg bw in rats.

Applicant's summary and conclusion

Interpretation of results:
toxic
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The LD50 of ammonium hydroxide in water was 350mg/kg bw in rats.
Executive summary:

The acute oral toxicity of ammonium hydroxide (aqueous ammonia) was investigated in the rat. Groups of ten male Wistar rtas were gavaged with a single oral dose of the the substance dissolved in water at various dose levels and observed for 14 days. The LD50 was calculated to be 350 mg/kg bw using the probit method.