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Ecotoxicological information

Toxicity to terrestrial plants

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Link to relevant study record(s)

Reference
Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Peer reviewed journal
Justification for type of information:
Data is from Peer reviewed journal
Qualifier:
according to guideline
Guideline:
ISO 11269-1 (Soil quality - Determination of the effects of pollutants on soil flora - Part 1: Method for the measurement of inhibition of root growth)
GLP compliance:
not specified
Analytical monitoring:
yes
Species:
Brassica oleracea var. capitata
Plant group:
other: Angiosperms
Details on test organisms:
- Common name: cabbage
- Source: state company EPAGRI (Empresa de Pesquisa Agropecua´ria e Difus~ao de Tecnologia de Santa Catarina, Floriano´polis, SC, Brazil).
Test type:
seed germination/root elongation toxicity test
Study type:
laboratory study
Substrate type:
natural soil
Total exposure duration:
16 d
Test temperature:
day-time temperature of 25 ± 2°C and a nighttime temperature of 16 ±2°C
Details on test conditions:
- Test vessel: pots
- No. of vessels per concentration (replicates): Three
- Material, size, headspace, fill volume:
disposable plastic
- No. of organisms per vessel: Twenty-five cabbage
-Other: 7-cm diameter
5-cm height of pots used

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h light:dark
- Light intensity: 60 mE/m2/s (cool-white fluorescent lamps)
Reference substance (positive control):
yes
Remarks:
Red-yellow Podzolic soil
Key result
Species:
Brassica oleracea var. capitata
Duration:
6 d
Dose descriptor:
LOEC
Effect conc.:
2 other: stimulant effect
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
germination
Species:
Brassica oleracea var. capitata
Duration:
16 d
Dose descriptor:
LOEC
Effect conc.:
4 other: stimulant effect
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Fresh biomass
Details on results:
LOEC value of test chemical for 6 and 16 days was observed to be 2 and 4 respectively on the basis of stimulant effect
Reported statistics and error estimates:
Statistical analysis was carried out on a microcomputer using the TOXSTAT 3.0 software.
Validity criteria fulfilled:
not specified
Conclusions:
LOEC value of test chemical for 6 and 16 days was observed to be 2 and 4 respectively on the basis of stimulant effect.
Executive summary:

The study was conducted to determine toxicity of test chemical to soil macro-organism in accordance with ISO guideline. Toxicity of test chemical to plant was performed by phytotoxicity tests which were conducted with Brassica oleracea L. var. capitata (cabbage). Seeds of the test plant were supplied by the state company EPAGRI. Twenty-five cabbage seeds were sown in each pot containing 200 g of soil or soil: sludge mixture. Test was performed in three pots of disposable plastics which were 7-cm in diameter and 5-cm height and each plant test was performed in duplicate. Plants were grown under controlled conditions at a day-time temperature of 25±2°C and a nighttime temperature of 16 ±2°C with lighting of 60 mE/m2/s under a 16:8 h light: dark photoperiod. Cool-white fluorescent lamps were used to provide light. Same red-yellow Podzolic soil used as The reference (control) soil in the soil: sludge mixtures. Sludge was mixed to the soil in different proportions of 50; 25; 12.5; 6.2; and 3.1% and dechlorinated tap water was gently pipetted onto the soil surface at 66% (i.e., 64 ml) of the maximum water-holding capacity (48 ml water/100 g soil). Statistical analysis of test chemical was carried out on a microcomputer using the TOXSTAT 3.0 software. LOEC value of test chemical for 6 and 16 days was observed to be 2 and 4 respectively on the basis of stimulant effect.

Description of key information

LOEC value of test chemical for 6 and 16 days was observed to be 2 and 4 respectively on the basis of stimulant effect.

Key value for chemical safety assessment

Additional information

Following study includes experimental study for the target chemical to observe the toxicity of test chemical to terrestrial plants as follows:

The study was conducted to determine toxicity of test chemical to soil macro-organism in accordance with ISO guideline. Toxicity of test chemical to plant was performed by phytotoxicity tests which were conducted with Brassica oleracea L. var. capitata (cabbage). Seeds of the test plant were supplied by the state company EPAGRI. Twenty-five cabbage seeds were sown in each pot containing 200 g of soil or soil: sludge mixture. Test was performed in three pots of disposable plastics which were 7-cm in diameter and 5-cm height and each plant test was performed in duplicate. Plants were grown under controlled conditions at a day-time temperature of 25±2°C and a nighttime temperature of 16 ±2°C with lighting of 60 mE/m2/s under a 16:8 h light: dark photoperiod. Cool-white fluorescent lamps were used to provide light. Same red-yellow Podzolic soil used as The reference (control) soil in the soil: sludge mixtures. Sludge was mixed to the soil in different proportions of 50; 25; 12.5; 6.2; and 3.1% and dechlorinated tap water was gently pipetted onto the soil surface at 66% (i.e., 64 ml) of the maximum water-holding capacity (48 ml water/100 g soil). Statistical analysis of test chemical was carried out on a microcomputer using the TOXSTAT 3.0 software. LOEC value of test chemical for 6 and 16 days was observed to be 2 and 4 respectively on the basis of stimulant effect.