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The biodegradability of charcoal (Probe 2: C-Fix = 80.5%) was tested according to OECD guideline No. 301B (CO2 evolution test) and under GLP compliance.

The test item was tested in a concentration of 25 mg/L. As positive control, aniline was tested in parallel. The biodegradation of the test substance was determined by measuring the emitted CO2concentrations on test days 2, 4, 7, 9, 11, 14, 18, 23, during the 28-day test period and on day 29. The biodegradation for the whole test period was determined as <2%. Hence, charcoal (Probe 2: C-Fix = 80.5%) is considered not readily biodegradable. For the positive control, a degradation of 64% was achieved within 9 d, ensuring the validity of the test.