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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November 1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study not in GLP, purity of the sample has not been assessed, but the study is conducting following official guideline and scientifically valid to assess the end point
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
yes
Remarks:
Water accomodation fraction
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Solutions are prepared just before testing. In comparaison with the control, a series of geometric concentration solutions has been prepared to obtain 100% imobilisation after 48h.

WAF solutions are prepared: 10%, 1%, 0.1%, 0.01%, 0.001%
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna strauss of more than 6 hours and less than 24 hours old.
Source: U.G. University of Gand - lab. Prof Persoone
Alimentation: algae Selenastrum capricornutum
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
No
Test temperature:
20°C +/- 1°C
Nominal and measured concentrations:
10%, 1%, 0,1%, 0.01%, 0.001%
Details on test conditions:
Total obscuration
Not air, light or feeding during the test
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 2 504 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

20 organismas for any concentration divided in 4 goups of 5 animals each

Results table:

WAF concentration Imobilised daphnis after 48h % imobilised
A B C D
Control  0/5  0/5  0/5  0/5 0
WAF 10%  5/5  5/5  5/5  5/5 100
WAF 1%  4/5  5/5  4/5  5/5 80
WAF 0.1%  1/5  0/5  2/5  0/5 15
WAF 0.01%  0/5  0/5  0/5  0/5 0
WAF 0.001%  0/5  0/5  0/5  0/5 0
Validity criteria fulfilled:
not specified
Conclusions:
WAF EC50 48 Daphnia magna = 2504 mg/l
Executive summary:

The short term toxicity to aquatic invertebrates has been assessed following OECD 202 on rape oil methyl ester, with deviation for poor soluble substances following ISO 147.

Description of key information

WAF EC50 = 2504 mg/L

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
2 504 mg/L

Additional information