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EC number: 215-175-0 | CAS number: 1309-64-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro DNA damage and/or repair study
- Remarks:
- Type of genotoxicity: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The reference does not totally comply with the specific testing guideline: no positive controls or metabolic activation system were included. Due to the missing positive and negative control, the study was considered not reliable.
Data source
Reference
- Reference Type:
- publication
- Title:
- Rec assay and mutagenicity studies on metal compounds
- Author:
- Kanematsu N., Hara M. and Kada T.
- Year:
- 1 980
- Bibliographic source:
- Mutation Research, 77:109-116
Materials and methods
Test guideline
- Guideline:
- other: no guideline specified
- Principles of method if other than guideline:
- In this study where the genotoxicity of metal compounds were evaluated, antimony trioxide was tested in the Bacillus subtilis Rec assay .
- GLP compliance:
- not specified
- Type of assay:
- Bacillus subtilis recombination assay
Test material
- Reference substance name:
- Diantimony trioxide
- EC Number:
- 215-175-0
- EC Name:
- Diantimony trioxide
- Cas Number:
- 1309-64-4
- Molecular formula:
- Sb2O3
- IUPAC Name:
- oxostibanyl stibinate
- Test material form:
- not specified
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- bacteria, other: Bacillus subtilis H17 (Rec+, arg- try-) and M45 (Rec-, arg- try-)
- Test concentrations with justification for top dose:
- The antimony trioxide was dissolved in distilled water at a concentration of 0.001-10 M.
2.5 µmol (729 µg)/disk
Controls
- Untreated negative controls:
- no
- Positive controls:
- no
- Details on test system and experimental conditions:
- In this study the genotoxicity of 127 metal compounds were evaluated. One of the compounds was antimony trioxide that was tested in the Bacillus
subtilis Rec assay.
The strains were grown overnight in B-2 broth (meat extract 10g, polypeptone powder 10g, and NaCl 5 g in 1L, pH adjusted to 7.0) and conserved at -80°C with 12.5% glycerol supplemention. On the day of the experiment, each stock was melted and streaked radially from small pipettes onto
B-2 agar.
A 0.05 ml portion of the metal solution was dropped onto a filter paper disk (diameter 10 mm) and the disk was placed on the starting point of the
streak. The plates were kept at 4°C for 24 hours and then incubated at 37°C overnight.
When the DNA damage is produced by a chemical and subjected to cellular recombination-repair function, the growth of recombination-deficient
cells is usually inhibited much more than that of wild cells. - Evaluation criteria:
- When a metal compound has the capacity to damage DNA, growth of the Rec- strain of Bacillus subtilis must be more inhibited than that of the wild
strain. To predict the DNA-damaging capacity, the inhibition zones were measured and compared.
A difference in the inhibition length of more than 4 mm was judged as positive.
Results and discussion
- Additional information on results:
- The treatment with antimony trioxide resulted in a difference in inhibition length of 5 mm and was thereby classified as positive.
Any other information on results incl. tables
Table 1: Results of the Rec assay on positive metal compounds
metal compound |
concentration of solution (M) |
inhibition length (mm) |
difference |
conclusiona |
|
H17 (Rec+) |
M45 (Rec-) |
||||
Sb2O3 |
0.05 |
0 |
5 |
5 |
+ |
a + Less than 6 mm and more than 4 mm of difference. |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
The treatment with antimony trioxide resulted in a difference in inhibition length of 5 mm and was thereby classified as positive.
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