Registration Dossier
Registration Dossier
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EC number: 233-139-2 | CAS number: 10043-35-3
Ecotoxicological information
Long-term toxicity to fish
Currently viewing:
Administrative data
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Cross-reference
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E1241-05 Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1400 (Fish Early-life Stage Toxicity Test)
- GLP compliance:
- no
- Remarks:
- study is not in compliance with GLP
Test material
- Reference substance name:
- Boric acid
- EC Number:
- 233-139-2
- EC Name:
- Boric acid
- Cas Number:
- 10043-35-3
- Molecular formula:
- H3BO3
- IUPAC Name:
- boric acid
- Reference substance name:
- 1303-96-4
- EC Number:
- 603-411-9
- Cas Number:
- 1303-96-4
- IUPAC Name:
- 1303-96-4
- Reference substance name:
- Disodium tetraborate, anhydrous
- EC Number:
- 215-540-4
- EC Name:
- Disodium tetraborate, anhydrous
- Cas Number:
- 1330-43-4
- Molecular formula:
- Na2B4O7
- IUPAC Name:
- Disodium tetraborate
- Reference substance name:
- disodium tetraborate decahydrate
- IUPAC Name:
- disodium tetraborate decahydrate
- Details on test material:
- Mixture of 82% (weight) boric acid and 18% borax
Physical state: white powders
Analytical purity: Boric acid - reagent, ACS
Borax - 99.5+% for analysis ACS (Supplier: Acros organics via Fisher Scientific)
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Each treatment sampled at beginning and end of test
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Source: Aquatic Biosystems, Fort Collins Colorado
METHOD FOR PREPARATION AND COLLECTION OF FERTILIZED EGGS
- Numbers of parental fish (i.e. of females used to provide required number of eggs): done by supplier
- Method of collection of fertilised eggs: done by supplier
- Subsequent handling of eggs: Shipped via express delivery. Test begun upon arrival
POST-HATCH FEEDING
- Type/source of feed: brine shrimp
- Amount given:
- Frequency of feeding: 2x per day - days 0-2 and 5 days per week for rest of test. 1x per day on weekends (2 days per week)
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 32 d
Test conditions
- Hardness:
- 84 to 94
- Test temperature:
- 23.6 to 25.5
- pH:
- 7.5 to 8..2
- Dissolved oxygen:
- 5.13 to 7.5
- Nominal and measured concentrations:
- Nominal: control, 2.75, 5.5, 11, 22, 44 mg-B/L
Measured: 0.03, 2.8, 5.7, 11.2, 23, 44.5 mg-B/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: day 0-2 1-L beakers, days 2-32 600 ml beakers
- Type : open
- Material, size, headspace, fill volume: glass, day 0-2 1L, days 2-32 500 ml
- Aeration: days 0-2 only
- Renewal rate of test solution: 90% every 3 days
- No. of fertilized eggs/embryos per vessel: day 0-2 60/chamber, days 2-32 10/chamber
- No. of vessels per concentration (replicates): day 0-2 one per concentration, days 2-32 four per concentration
- No. of vessels per control (replicates): day 0-2 one per concentration, days 2-32 four per concentration
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water) per US EPA 2002
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46
- Conductivity:
- Salinity:
- Culture medium different from test medium: Aquatic Biosystems Lab water, hardness 114 mg/L, alkalinity 115 mg/L
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness on "in" and "out" water on every changeover, temperature and D.O. daily
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Survival (daily), dry weight per 10 fish (at conclusion of test)
RANGE-FINDING STUDY
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 11.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Duration:
- 32 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 23 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Duration:
- 32 d
- Dose descriptor:
- other: MATC
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 44.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- weight
- Remarks on result:
- other: (dry weight per 10 fish)
- Duration:
- 32 d
- Dose descriptor:
- LC10
- Effect conc.:
- 21.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks on result:
- other: confidence limits: 19.3-24.2 mg/L
- Reported statistics and error estimates:
- Data tested for normality using the Shapiro-Wilk's test, and for homogeneity of variance using Bartlett's test. Data passing both tests were analyzed for differences between group and control using Dunnett's test. Those not passing both tests were analysed using Steele's Many-One test.
The LC10/EC10 values have been recalculated with the log-logistic model using Toxicity Relationship Analysis Program, Version 1.21A
Any other information on results incl. tables
Suvival Data
| Day | Control | 2.8 | 5.7 | 11.2 | 23 | 44.5 |
| 3 | 100.0% | 100.0% | 100.0% | 100.0% | 100.0% | 100.0% |
| 7 | 100.0% | 97.5% | 95.0% | 97.5% | 95.0% | 97.5% |
| 14 | 100.0% | 92.5% | 87.5% | 95.0% | 92.5% | 85.0% |
| 21 | 97.5% | 92.5% | 87.5% | 95.0% | 87.5% | 42.5% |
| 28 | 95.0% | 92.5% | 87.5% | 95.0% | 80.0% | 20.0% |
| 32 | 95.0% | 92.5% | 87.5% | 95.0% | 80.0% | 15.0% |
Dry weight data (mg)
| Day | Control | 2.8 | 5.7 | 11.2 | 23 | 44.5 |
| 32 | 0.8926 | 0.9336 | 0.7946 | 0.8595 | n/a | n/a |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
