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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro cytogenicity / chromosome aberration study in mammalian cells
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Primary mutagenicity screening of food additives currently used in Japan
Author:
Ishidate, M., Jr., Sofumi, T., Yoshikawa, K., Hayashi, M.,|Nohmi, T., Sawada, M. and Matsuoka, A.
Year:
1984
Bibliographic source:
Fd Chem. Toxic., 22, 623-636

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
Deviations:
yes
Remarks:
no test with metabolic activation
GLP compliance:
not specified
Type of assay:
in vitro mammalian chromosome aberration test

Test material

Constituent 1
Chemical structure
Reference substance name:
Calcium chloride
EC Number:
233-140-8
EC Name:
Calcium chloride
Cas Number:
10043-52-4
Molecular formula:
CaCl2
IUPAC Name:
calcium dichloride
Details on test material:
No data.

Method

Species / strain
Species / strain / cell type:
other: Chinese hamster fibroblast cell line CHL
Metabolic activation:
without
Test concentrations with justification for top dose:
max. 4 mg/plate

Results and discussion

Test results
Species / strain:
Chinese hamster lung fibroblasts (V79)
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks on result:
other: all strains/cell types tested

Applicant's summary and conclusion