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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Taken from OECD SIDS (Guideline study).
Principles of method if other than guideline:
Method: other: EPA/600/4-90/027, EPA/600/6-91/003
GLP compliance:
not specified
Analytical monitoring:
not specified
Test organisms (species):
Pimephales promelas
Details on test organisms:
a) Size: Not described
b) Age: 1-7 days old
c) Pretreatment: no data
d) Supplier/Source: ENSR, Fort Collins, CO, USA
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
96 h
Dose descriptor:
Effect conc.:
4 630 mg/L
Basis for effect:
mortality (fish)
48 h
Dose descriptor:
Effect conc.:
> 6 560 mg/L
Basis for effect:
mortality (fish)
24 h
Dose descriptor:
Effect conc.:
> 6 660 mg/L
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:
Table: Mean LC50 values

Exposure period 24h 48h 96h
Number of test 3 3 3
LC50 (mg/l)* >6660 >6560 4630
Range (mg/l)** 4700->10000 4390->10000 3930-5360

* Values are arithmetic mean of 3 tests.
** Range of three LC50s

Description of key information

Eight studies on acute toxicity to fish have been reported, of which 4 were considered reliable with restrictions (K2). These are presented in the current summary. Four other studies were scored K3 and are not further discussed.

The key study (Mount et al. 1997, performed under EPA guideline) estimated the lowest 96-hour LC50 value of 4630 mg/L as result of exposing fathead minnow (Pimephales promelas). The other 3 K2 studies exposing bluegill sunfish (Lepomis macrochirus) (Cairns and Scheier (1959) and Trama (1954)) and mosquitofish (Gambusia affinis) (Wallen et al. 1957) resulted in 96-hour LC50 values between 9500 and 13400 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
4 630 mg/L

Additional information