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EC number: 215-535-7 | CAS number: 1330-20-7
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, non-guideline in-vitro and animal experimental study, published in peer reviewed literature, minor restrictions in reporting but otherwise adequate for assessment.
Cross-reference
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 005
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 428 (Skin Absorption: In Vitro Method)
- Principles of method if other than guideline:
- Skin penetration and skin retention studies (with mass balance determination) were conducted.
- GLP compliance:
- no
Test material
- Reference substance name:
- mixed xylenes
- EC Number:
- 924-522-1
- IUPAC Name:
- mixed xylenes
- Reference substance name:
- Xylene
- EC Number:
- 215-535-7
- EC Name:
- Xylene
- Cas Number:
- 1330-20-7
- Molecular formula:
- C8H10
- IUPAC Name:
- xylene
- Details on test material:
- - Name of test material (as cited in study report): xylene
- Molecular formula: C6H6-(CH3)2
- Molecular weight: 106.17
- Substance type: hydrocarbon
- Isomers composition: no data
- Radiochemical purity (if radiolabelling): 98.0%
- Melting point: -25.2°C
- Boiling point: 144.5 °C
- Log Kp: 3.12
- Water solubility (mg/L): 178
Constituent 1
Constituent 2
- Radiolabelling:
- yes
Test animals
- Species:
- rat
- Strain:
- other: CD
- Sex:
- not specified
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Duration of exposure:
- 8 hr
- Doses:
- - Dose volume: 0.5 ml containing 2.5 µCi per cell
- No. of animals per group:
- 9
- Control animals:
- no
- Details on in vitro test system (if applicable):
- Skin penetration and skin retention studies (with mass balance determination) were conducted.
Results and discussion
- Absorption in different matrices:
- The in-vitro dermal penetration of xylene through rat skin was 0.224% in 8 hours.
- Total recovery:
- 94.21±2.61% at 8h.
Percutaneous absorption
- Parameter:
- percentage
- Absorption:
- 0.2 %
- Remarks on result:
- other: 8h
- Remarks:
- The flux of xylene through rat abdominal skin was 0.0037 mg/cm2/min
Any other information on results incl. tables
Xylene showed a lag time of about 1 h. Retention in the stratum corneum was much higher than epidermis and dermis at all time points. Under infinite dose conditions, xylene diffused rapidly into the stratum corneum and reached plateau levels within 3 h. The recovery (mass balance) for xylene was 92-105%. This indicated a minimum evaporation of chemical during the skin retention study.
Applicant's summary and conclusion
- Conclusions:
- The in-vitro dermal penetration of xylene through rat skin was 0.224% in 8 hours.
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