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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study not in GLP, purity of the sample has not been assessed, but the study is conducting following official guideline and scientifically valid in assessing the end point
equivalent or similar to guideline
other: ISO 10712
not specified
GLP compliance:
Analytical monitoring:
Details on test solutions:
The sample is poor water soluble: the protocol ISO 147 has been followed. Saturated solutions have been prepared, the more concentrated at 10% (10g/100ml).
Starting from that, following dilution have been prepared: 8%, 1%, 0.1%, 0.01%, 0.001%
Test organisms (species):
Pseudomonas putida
Details on inoculum:
-Age of the culture stock: 7 days
-Age of pre-colture: 5+/- h at 25°C
Test type:
Water media type:
Limit test:
Total exposure duration:
16 h
Key result
16 h
Dose descriptor:
Effect conc.:
ca. 5 250 mg/L
Nominal / measured:
Conc. based on:
other: WSF: Water Soluble Fraction
Basis for effect:
growth inhibition
Reported statistics and error estimates:
Calculation mehtod: Trimmed Spearman-Karber method

Concentration Absorbtion 0h Absorption 16h Difference % Inhibition
Control 0.015 0.101 0.086  - 
WSF 8% 0.015 0.088 0.073 15
WSF 1% 0.01 0.093 0.08 3
WSF 0.1% 0.01 0.10 0.089 0
WSF 0.01% 0.01 0.097 0.087 0
WSF 0.001% 0.01 0.099 0.089 0
WSF EC0 16h Pseudonomas putida = 5250 mg/l
Executive summary:

The fatty acid, rape oil, methyl ester has been tested for The inhibition of the growth of Pseudonomas Putida following ISO 10712 and ISO 147 for poorly soluble substances

Description of key information

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
5 250 mg/L

Additional information