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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Final report, July 1976
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP, similiar to standard guideline, published, minor restrictions in design and/or reporting but otherwise adequate for assessment
Qualifier:
equivalent or similar to guideline
Guideline:
other: Macroinvertebrate and fish toxicity tests followed the recommended bioassay procedures as described in the “Methods for Acute Toxicity Tests with Fish, Macro invertebrates, and Amphibians” (US EPA 1975)
Deviations:
not specified
Principles of method if other than guideline:
Macroinvertebrate and fish toxicity tests followed the recommended bioassay procedures as described in the “Methods for Acute Toxicity Tests with Fish, Macro invertebrates, and Amphibians” (US EPA 1975)
GLP compliance:
no
Specific details on test material used for the study:
Dicyclopentadiene (DCPD): clear liquid received from Aldrich Chemical company, Milwaukee, Wisconsin. The DCPD was tested on a 95% active ingredient basis.
Analytical monitoring:
yes
Details on sampling:
Dissolved oxygen contration, pH and temperature of test solutions were checked at 0, 48 and 96 hrs in 2 selected test concentrations at a minimum.
Vehicle:
yes
Remarks:
acetone was used as a solvent/carrier
Details on test solutions:
Stock solutions were prepared in a solution of reagent-grade acetone. Stock solution for fish ration of 1.5 parts DCPD : 98.5 parts acetone (volume:volume). Negative controls, consisting of the same dilution water and conditions as test concentrations but no DCPD
Test organisms (species):
other: Ictalurus punctatus
Details on test organisms:
Mean wet weight of channel catfish was 1.3 g.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
N/A
Post exposure observation period:
not reported
Hardness:
not reported
Test temperature:
21 ± 1.0 °C for channel catfish.
pH:
6.9 - 7.3
Dissolved oxygen:
3.8 - 8.8 mg/L
Salinity:
not reported
Conductivity:
N/A
Nominal and measured concentrations:
nominal concentrations (mg/L): control, control (acetone), 14.0, 16.0, 18.0, 24.0, 32.0
Details on test conditions:
Static fish bioassays were conducted in 19.6 L glass vessels held in contact temperature water baths at 21±1.0.°C for the channel catfish. The standard diluents (well water) used had the same water quality characteristics as that for holding water.
Reference substance (positive control):
yes
Remarks:
control (acetone)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
15.7 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
not specified
Details on results:
Reported as median lethal concentration
Results with reference substance (positive control):
Results showed in the table included in section "Any other information on results inc. table" show no mortality in the control (acetone) group
Reported statistics and error estimates:
95% confidence levels
Sublethal observations / clinical signs:

Table: Concentrations tested and corresponding observed percentage mortalities for channel catfish (Ictalurus punctatus) exposed to dicyclopentadiene ( DCPD) for 24 , 48 and 96 hours .

 Species   Nominal concentration (mg/L)  % motality observed      
 24 h  48 h  96 h
   Control  0  0  0
   Control (acetone)  0  0  0
   14.0  0  0  0
   16.0  0  40  60
   18.0  20  20  100
   24.0  60  60  100
   32.0  100  100  100
Validity criteria fulfilled:
not applicable
Remarks:
This is a non standard study
Conclusions:
This is a 96 h LC50 experimental study, non GLP and similar to standard guidelines, with notable restrictions in design and/or reporting but contributing to weight of evidence assessment. The 96 h LC50 Ictalurus punctatus was reported to be 15.7 mg/L.
Executive summary:

This is a 96 h LC50 experimental study, non-GLP and similar to standard guidelines, with notable restrictions in design and/or reporting but contributing to weight of evidence assessment. The 96 h LC50 Ictalurus punctatus was reported to be 15.7 mg/L

Description of key information

The 96 hour LC50 is 15.7 mg/L. The estimated LC50 value from TLM QSAR is 6.712 mg/L.

The experimental value was used for the chemical safety assessment.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Remarks:
96 hour LC50
Effect concentration:
15.7 mg/L

Additional information

An Environmental Agency Japan test following OECD guideline 203, using semi-static conditions without analytical confirmation of exposure concentrations showed the 96-hour LC50 to be 4.3 mg/L. This study was unavailable for review but was included in the OECD SIDS for DCPD.

Bentley et al (1976) tested the toxicity of dicyclopentadiene to four species of fish. The range of LC50 values reported within the paper was between 15.7 -31.1 mg/L. The study followed a method similar to standard guidelines but did not include analytical confirmation of the exposure concentrations.

A QSAR result has also been included as part of the weight of evidence, which used the ECOSAR program to derive a 96-hour LC50 of 9.8 mg/L and Target Lipid modelling to derive an estimated LL50 for this substance is 6.712 mg/L.

The lowest of the measured results which are considered to be reliable has been conservatively selected as the key value for this endpoint.

The 96-hour LC50 for freshwater fish is 15.7 mg/L (Bentley et al 1976).