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EC number: 232-391-0 | CAS number: 8013-07-8
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22/09/88 - 20/10/88
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Not much information on full method. May not be GLP compliant. Very minor deviations
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- Please see Overall Remarks
- Principles of method if other than guideline:
- Determination of the biodegradability by measurement of the carbon dioxide formation in per cent of ThCO2 calculated from the ThOC or TOC
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Epoxidized soybean oil
- IUPAC Name:
- Epoxidized soybean oil
- Reference substance name:
- Soybean oil, epoxidized
- EC Number:
- 232-391-0
- EC Name:
- Soybean oil, epoxidized
- Cas Number:
- 8013-07-8
- Molecular formula:
- Not necessary, substance is a UVCB.
- IUPAC Name:
- Soybean oil, epoxidized
- Reference substance name:
- Reoplast 392 (Reoplast 39)
- IUPAC Name:
- Reoplast 392 (Reoplast 39)
- Reference substance name:
- TK 11278
- IUPAC Name:
- TK 11278
- Details on test material:
- - Name of test material (as cited in study report): TK 11278
- Physical state: Liquid
- Analytical purity: comm. grade
- Lot/batch No.: 22.07.88
- Stability under test conditions: 08/93
- Storage condition of test material: At room temperature
- Solubility: insoluble in water
Constituent 1
Constituent 2
Constituent 3
Constituent 4
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not relevant
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Bacteria were collected from activated sludge from a sewage treatment plant located in Reinhach
- Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10E05PO (solution of 31.2 mg in 100 ml bidist. water) and 15 rsp. 30 mg of test substance were added and homogenised. This solution was given to the test vessel which was immediately connected to the CO2 traps.
10 mg test substance are equivalent to 7.12 mg organic carbon.
- Test temperature: 22 ± 2 °C
- Aeration of dilution water: 25 ml/min of carbon dioxide free air
TEST SYSTEM
- Culturing apparatus: 2 L flasks equipped with gas inlet and magnetic stirrer
SAMPLING
Determination of the initial CO2 of the 0.05N sodium hydroxide and the CO2 absorbed in the absorbers filled with 200 ml 0.05N sodium hydroxide on the days 5, 11, 18, 22, 27 and 28.
CONTROL AND BLANK SYSTEM
Blank + Vehicle: Water as specified in the guideline containing 0.5 ml of the Nonylphenol solution (for calculation of biodegradation).
Blank: Water as specified in the guideline.
STATISTICAL METHODS:
The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the cumulative quantities of carbon dioxide determined on the days of measurements.
Reference substance
- Reference substance:
- aniline
- Remarks:
- 20 mg/L, MERCK No.: 1261
Results and discussion
- Preliminary study:
- No results stated
- Test performance:
- Not stated
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 79
- Sampling time:
- 28 d
- Remarks on result:
- other: 10 mg test substance/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 92
- Sampling time:
- 28 d
- Remarks on result:
- other: 20 mg test substance/L
- Details on results:
- The biodegradation calculated as percentage of measured amount of CO2 over the theory was:
10 mg test substance/L = 79 % in 28 days
20 mg test substance/L = 92 % in 28 days
Please see table 1 for further details
Therefore, TK11278 is readily biodegradable.
BOD5 / COD results
- Results with reference substance:
- The biodegradation was calculated as: 20 mg/L = 94.4 % in 28 days
Any other information on results incl. tables
Theoretical CO2 formation:
Reference Substance: 20 mg/L = 85.061 mg CO2/1.5L
Test Substance: 10 mg/L = 39.13 mg CO2/1.5L
Test Substance: 20 mg/L = 78.26 mg CO2/1.5L
Table 1: CO2 Evolution and Biodegradation
DAY |
Blank |
Blank and vehicle |
Aniline (Ref. Substance) 20 mg/L
|
Test Substance at 10 mg/L |
Test Substance at 20 mg/L |
|||||||||||
CO2 Evolution |
CO2 Evolution |
CO2 Evolution |
Biodegradation |
CO2 Evolution |
Biodegradation |
CO2 Evolution |
Biodegradation |
|||||||||
[mg] |
[mg]* |
[mg] |
[mg]* |
[mg] |
[mg]* |
[%] |
[%]* |
[mg] |
[mg]* |
[%] |
[%]* |
[mg] |
[mg]* |
[%] |
[%]* |
|
5 |
2.7 |
2.7 |
4.2 |
4.2 |
16.1 |
16.1 |
19.0 |
19.0 |
16.3 |
16.3 |
41.8 |
42 |
42.1 |
42.1 |
53.8 |
54 |
11 |
6.4 |
9.2 |
6.1 |
10.3 |
47.3 |
63.4 |
55.6 |
74.5 |
10.3 |
26.7 |
26.4 |
68 |
19.0 |
61.1 |
24.3 |
78 |
18 |
7.6 |
16.8 |
7.7 |
18.0 |
7.2 |
70.6 |
8.4 |
83.0 |
3.0 |
29.7 |
7.7 |
76 |
6.5 |
67.6 |
8.3 |
86 |
22 |
3.7 |
20.4 |
4.0 |
22.0 |
3.8 |
74.4 |
4.5 |
87.4 |
-0.4 |
29.3 |
-0.9 |
75 |
0.1 |
67.7 |
0.1 |
87 |
27 |
7.3 |
27.7 |
5.6 |
27.6 |
5.9 |
80.2 |
6.9 |
94.3 |
1.5 |
30.9 |
3.9 |
79 |
3.4 |
71.2 |
4.4 |
91 |
28 |
1.2 |
28.9 |
1.7 |
29.3 |
0.1 |
80.3 |
0.1 |
94.4 |
-0.1 |
30.8 |
-0.2 |
79 |
1.2 |
72.3 |
1.5 |
92 |
* = cumulative values
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- TK11278 is readily biodegradable.
- Executive summary:
A ready biodegradability test (OECD guideline 301B) was conducted on TK 11278 (otherwise known as Reoplast 39 or epoxidised soybean oil) using bacteria from a sewage treatment plant. Aniline was used as a reference substance.
After 28 days:
The biodegradation calculated as percentage of measured amount of CO2 over the theory was:
10 mg test substance/L = 79 % in 28 days
20 mg test substance/L = 92 % in 28 days
The % degradation (% ThCO2 formation) exceeded 10% within 2 to 3 days for both concentrations of the test material and subsequently exceeded the 60% pass level within the 10 day window.
Therefore, epoxidised soybean oil is readily biodegradable.
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