Cyclohexanone

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Principles of method if other than guideline:

BASF-internal standards; estimation of the LC50 value

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexanone

EC Number:

203-631-1

EC Name:

Cyclohexanone

Cas Number:

108-94-1

Molecular formula:

C6H10O

IUPAC Name:

cyclohexanone

Specific details on test material used for the study:

purity: 99.9%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Body weight range of the animals: 185 ± 15 g
The animals were offered Herilan MRH of H. EGGERSMANN KG, Rinteln/Weser, and tap water ad libitum during the post-exposure observation period.
The animals were kept in fully air-conditioned rooms (temperature 22 ± 2°C and humidity 55 ± 5%) with a day/night rhythm of 12 hours.

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Details on inhalation exposure:

A concentration estimated on the basis of the data from the inhalation hazard test was used as a first orientating concentration. One concentration allows a no effect level to be established (0 lethality).

Duration of exposure:

4 h

Concentrations:

6.2 mg/l

No. of animals per sex per dose:

10

Control animals:

yes

Details on study design:

The body weight of the animals was checked before the beginning of the test, after 7 days and at the end of the observation period. Clinical findings and lethality were recorded daily.
At the end of the 14-day observation period, the animals were sacrificed with CO2 and were subjected to a gross-pathological examination like all other animals which had died before.

Statistics:

Statistical evaluation of the test were done according to the binomal test (BASF SE).

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 6.2 mg/L air

Exp. duration:

4 h

Remarks on result:

other: significance level: 1%

Mortality:

none

Clinical signs:

other: Watery eye and nose secretion, mouth smearing, intermittent and accelerated breathing, apathetic, narcosis, scrubby fur. Three of 10 female animals showed alopecia. Symptoms were seen until day of killing.

Body weight:

No significant differences to the control group.

Gross pathology:

Sacrificed animals: nothing abnormal detected.

Any other information on results incl. tables

1) LC50 (4 h) for male and female animals: = > 6.2 mg/l air

2) LC50 (1 h) for male and female animals: = > 25 mg/l air*

3) LC50 (4 h) for male animals: = > 6.2 mg/l air

4) LC50 (1 h) for male animals: = > 25 mg/l air*

5) LC50 (4 h) for female animals: = > 6.2 mg/l air 6) LC50 (1 h) for female animals: = > 25 mg/l air*

* By means of converting the values of the 4-hour LC50 according to the rule of Haber C x t = k

Applicant's summary and conclusion

Interpretation of results:

other: The test substance is labelled with R20 (Directive 67/548/EEC)