Registration Dossier

Toxicological information

Specific investigations: other studies

Currently viewing:

Administrative data

Endpoint:
specific investigations: other studies
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Reliability established by secondary source. Only secondary source evaluated.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Comparative study of the acute lung toxicity of pure cobalt powder und cobalt-tungsten carbide mixture in rat.
Author:
Lasfargues G, Lison D, Maldague P and Lauwerys R.
Year:
1992
Bibliographic source:
Toxicol Appl Pharmacol 112, 41-50.
Reference Type:
secondary source
Title:
SIDS Initial Assessment Report for SIAM 21 for Tungsten Carbide (12070-12-1), Washington DC,18-20 October, 2005
Author:
OECD-SIDS
Year:
2005
Bibliographic source:
UNEP Publications

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Acute lung effects of tungsten carbide were evaluated after intratracheal installation of tungsten carbide in saline.
GLP compliance:
not specified
Type of method:
in vivo

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity: > 99%
Median particle diameter: 2 um.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
female
Details on test animals and environmental conditions:
no data

Administration / exposure

Route of administration:
intratracheal
Vehicle:
physiological saline
Details on exposure:
no data
Analytical verification of doses or concentrations:
not specified
Details on analytical verification of doses or concentrations:
no data
Duration of treatment / exposure:
(2) single injections
Post exposure period:
no data
Doses / concentrationsopen allclose all
Remarks:
Doses / Concentrations:
Experiment 1: 157 mg/kg bw
Basis:
no data
Remarks:
Doses / Concentrations:
Experiment 2: 10 mg/kg bw
Basis:
no data
No. of animals per sex per dose:
Experiment 1: 10 females
Experiment 2: 5 females
Control animals:
yes
Details on study design:
Experiment 1: 10 female Sprague-Dawley rats were instilled intratracheally with a tungsten carbide suspension of 157 mg/kg bw in physiological saline. 48 hours later animals were killed and lungs were removed.

Examinations

Examinations:
no data
Positive control:
no

Results and discussion

Details on results:
Experiment 1: Histopathological examination of lungs showed that 157 mg/kg bw tungsten carbide behaved as an inert dust producing only a mild accumulation of macrophages in the alveolar duct walls.

Experiment 2: Cellular and biochemical characteristics of bronchoalveolar lavage fluid at 10 mg/kg bw were not significantly different from those of control animals.

Applicant's summary and conclusion