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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
GLP guideline study. Reliability assigned by a secondary source (OECD SIDS Initial Assessment Report). Only secondary source reviewed, as primary study was not available.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
yes
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: STRAUSS
- Source: Federal Health Office, Berlin Germany
- Age at study initiation (mean and range, SD): 2-24 hours.
- Feeding during test: None

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
N/A
Test temperature:
21 +/- 1 degrees C.
pH:
7.5 - 7.9
Dissolved oxygen:
9 mg/L
-The oxygen saturation was >= 60 % (21 degrees C) at the end of testing.
Nominal and measured concentrations:
32, 58, 100, 180, 320, 580, 1000 mg/L.
Details on test conditions:
TEST SYSTEM:
-Keeping water: M4 medium according to Elendt/Federal Health Office (BGA), Berlin Germany (1992).
-Photoperiod: 10 hours light.
-Illumination: diffuse light, illumination strength 1.5 – 5 umol*m-2*s-1.
-Test vessel: glass beakers, volume 50 mL, 20 mL test medium, not ventilated.
-Number of daphnia per beaker: 5 daphnids.
-Number of replicate: 4 plus 1 control.
-Test parameter: immobilization.
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
580 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 1 000 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Details on results:
The test substance was not soluble in dilution water in a concentration of 1000 mg/l. The test substance was deposited on the ground.

The recovery rates of the active ingredient at the end of the test were > 80 %. For that reason effect level concentrations are given as nominal concentrations.
The percentage of immobility was determined in all concentration groups and controls after 24 and 48 h. Effects - 24h-EC0 = 1000 mg/L
- 48h-EC0 = 580 mg/L
None of the test concentrations resulted in 100 % incapability of swimming during the test period:
- 24h-EC100 = >1000 mg/L
- 48h-EC100 = >1000 mg/L
From the test results a 48h-EC50 of >1000 mg/L was derived.

None of the control daphnids were incapable of swimming at the end of the test or swam on the surface
Reported statistics and error estimates:
no data
Conclusions:
48 hour EC50 >1000 mg/L

Description of key information

Tungsten carbide was tested for acute toxicity to aquatic invertebrates. In an immobilisation test using Daphnia magna and testing tungsten carbide, the 48h EC50 was found to be >1000 mg/L (938.7 mg W/L).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1 000 mg/L

Additional information

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