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EC number: 215-524-7 | CAS number: 1328-53-6 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 74260.
- Life Cycle description
- Uses advised against
- Endpoint summary
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- Storage stability and reactivity towards container material
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well documented study, sufficient for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Code of Federal Regulations, Title 16, Section 1500, 42
- Principles of method if other than guideline:
- This test was used for eye irritation test before OECD guideline 405 was established. It is generally similar to the Draize test, with the following deviations: 100 mg of the test substance was applied to the right eye (OECD recommends a volume of 0.1 ml), 6 animals were used (OECD: 3); observation period was 7 days (OECD: 21).
- GLP compliance:
- no
Test material
- Reference substance name:
- Polychloro copper phthalocyanine
- EC Number:
- 215-524-7
- EC Name:
- Polychloro copper phthalocyanine
- Cas Number:
- 1328-53-6
- Molecular formula:
- C32HxClyCuN8
- IUPAC Name:
- [1,2,3,4,8,9,10,11,15,16,17,18,22,23,25-pentadecachloro-5,26-dihydro-29H,31H-phthalocyaninato(2-)-kappa~2~N~29~,N~31~]copper
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- - Analytical purity: ca. 98 %
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: at the start of the test, the animals weighed ca. 2.5 kg
- Housing: Rabbits were kept in individual cages (540 x 360 x 315 mm) or in restraining devices
- Diet: 200 g of food per animal per day (Granules Lapin "entretien", Sanders)
- Water: softened and filtrated water ad libitum, automatically given
ENVIRONMENTAL CONDITIONS
- Temperature (°C): minimum 20 °C
- Air changes: ventilation (renewal of air 12 times per hour)
- Photoperiod (hrs dark / hrs light): 12/12
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: The left eye was treated with 50 mg talcum powder and served as control.
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied: 100 mg
- Concentration: undiluted - Duration of treatment / exposure:
- 24 h; the substance was not washed out.
- Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- 6
- Details on study design:
- 100 mg of the undiluted test substance was applied to the right conjuntival sac of 6 New Zealand White rabbits. The left eye was treated with 50 mg talcum powder and served as control. The substance was not washed out.
Ocular reactions were recorded at 1 h, 24 h, 48 h, 72 h, 96 h and 7 days after instillation using a scoring system, which is convertible into the current OECD Draize scheme. For the evaluation, the scores given in the raw data were converted to Draize scores. The reading time points used for assessment were 24, 48 and 72 hours after application.
Observations of the condition of the cornea were made with the naked eye and also with the aid of an ophtalmoscope of Heine.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Remarks:
- of 6 animals
- Time point:
- other: 24-48-72 h
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 h
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- of 6 animals
- Time point:
- other: 24-48-72 h
- Score:
- 0.11
- Max. score:
- 2
- Reversibility:
- fully reversible within: 72 h
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Remarks:
- of 6 animals
- Time point:
- other: 24-48-72 h
- Score:
- 0.11
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 h
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Remarks:
- of 6 animals
- Time point:
- other: 24-48-72 h
- Score:
- 0.06
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 h
- Irritant / corrosive response data:
- The following effects were observed, but were completely reversible within 72 hours:
- Test animals showed slight conjunctiva chemosis and redness, which were completely reversible in 5/6 animals within 48 hours;
- Iris congestion was also observed in 3/6 animals and was also completely reversible within 48 hours in 2 of the animals concerned;
- The cornea was slightly affected in 1/6 animals at 24 h only.
Any other information on results incl. tables
Table 1: Eye irritation parameters according to the method of Draize
Reading time point |
Animal 1, treated eye (right) |
||||
Conjunctiva Chemosis |
Conjunctiva Redness |
Iris score |
Cornea score |
Additional findings |
|
1 h |
1 |
1 |
1 |
0 |
|
24 h |
1 |
0 |
0 |
0 |
|
48 h |
1 |
0 |
0 |
0 |
|
72 h |
0 |
0 |
0 |
0 |
|
96 h |
0 |
0 |
0 |
0 |
|
7 d |
0 |
0 |
0 |
0 |
|
Reading time point |
Animal 2, treated eye (right) |
||||
Conjunctiva Chemosis |
Conjunctiva Redness |
Iris score |
Cornea score |
Additional findings |
|
1 h |
1 |
0 |
1 |
0 |
|
24 h |
0 |
0 |
0 |
0 |
|
48 h |
0 |
0 |
0 |
0 |
|
72 h |
0 |
0 |
0 |
0 |
|
96 h |
0 |
0 |
0 |
0 |
|
7 d |
0 |
0 |
0 |
0 |
|
Reading time point |
Animal 3, treated eye (right) |
||||
Conjunctiva Chemosis |
Conjunctiva Redness |
Iris score |
Cornea score |
Additional findings |
|
1 h |
1 |
1 |
0 |
0 |
|
24 h |
2 |
1 |
1 |
1 |
discharge |
48 h |
0 |
1 |
1 |
0 |
|
72 h |
0 |
0 |
0 |
0 |
|
96 h |
0 |
0 |
0 |
0 |
|
7 d |
0 |
0 |
0 |
0 |
|
Reading time point |
Animal 4, treated eye (right) |
||||
Conjunctiva Chemosis |
Conjunctiva Redness |
Iris score |
Cornea score |
Additional findings |
|
1 h |
1 |
1 |
0 |
0 |
|
24 h |
0 |
0 |
0 |
0 |
|
48 h |
0 |
0 |
0 |
0 |
|
72 h |
0 |
0 |
0 |
0 |
|
96 h |
0 |
0 |
0 |
0 |
|
7 d |
0 |
0 |
0 |
0 |
|
Reading time point |
Animal 5, treated eye (right) |
||||
Conjunctiva Chemosis |
Conjunctiva Redness |
Iris score |
Cornea score |
Additional findings |
|
1 h |
1 |
0 |
1 |
0 |
|
24 h |
1 |
0 |
0 |
0 |
|
48 h |
0 |
0 |
0 |
0 |
|
72 h |
0 |
0 |
0 |
0 |
|
96 h |
0 |
0 |
0 |
0 |
|
7 d |
0 |
0 |
0 |
0 |
|
Reading time point |
Animal 6, treated eye (right) |
||||
Conjunctiva Chemosis |
Conjunctiva Redness |
Iris score |
Cornea score |
Additional findings |
|
1 h |
1 |
0 |
0 |
0 |
|
24 h |
1 |
0 |
0 |
0 |
|
48 h |
0 |
0 |
0 |
0 |
|
72 h |
0 |
0 |
0 |
0 |
|
96 h |
0 |
0 |
0 |
0 |
|
7 d |
0 |
0 |
0 |
0 |
|
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
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