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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The substance has been shown to be readily biodegradable according to the test method described in the OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

A GLP study conducted according the international guideline OECD 301 B is available.

The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2produced by the respiration of bacteria. The degradation was stopped on day 28 by acidification of the test solutions. The last titration was made on day 29, after residual CO2had been purged from the test solutions over a period of 24 hours. The percentage CO2production was calculated in relation to the theoretical CO2production (ThCO2) of the test item. The biodegradation was calculated for each titration time.

To check the activity of the test system, sodium benzoate was used as functional control. The percentage degradation of the functional control reached the pass level of 60 % within 9 days and a biodegradation of 74 % after 28 days.

In the toxicity control containing both test and reference item a biodegradation of 62 % was determined within 14 days and it came to 79 % after 28 days. The biodegradation of the reference item was not inhibited by the test itemin the toxicity control.

The mean 10 % level (beginning of biodegradation) was reached on day 5. The 60 % pass level was reached after 25 days. The mean biodegradation after 28 days was 66

The test item is classified as readily biodegradable within 28 days.