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Long-term toxicity to fish

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Description of key information

Pimephales promelas: NOEC = 0.39 mg/L

Key value for chemical safety assessment

Additional information

The lowest, valid NOEC was derived from a study by Russom and Broderius (1991). The authors conducted early-life stage studies with Pimephales promelas with a variety of chemical substances, among them aniline. Embryos < 24 h old were exposed in a flow-through system to aniline for 32 days. The test substance concentration was measured at least twice a week. Test endpoints included percentage of normal larvae at hatch, percent survival and growth effects. A "chronic value", i.e the geometric mean of the NOEC and the LOEC, of 0.557 mg/I was found for the most sensitive endpoint, wet weight and total length. From this a NOEC of 0.39 mg/l can be derived according to the TGD.

A 28-day early-life-stage test with Brachydanio rerio was conducted by van Leeuwen et al. (1990). Fertilised eggs were exposed to aniline in a semistatic manner about 1 day after spawning. Test solutions were renewed three times a week. The actual aniline concentration was analysed before and after renewal of the test solution. It was found that the measured aniline concentrations were far below the nominal concentrations. A 28-day NOEC of 1.8 mg/1 for survival, hatching and growth and a 28-day NOLC (no observed lethal concentration) of 5.6 mg/l was found. Both values are based on nominal concentrations and are therefore not highly reliable. As no further information is given on the measured aniline concentration it is not possible to take into account the decreasing of the substance for the interpretation of the results. Therefore the NOEC from this study cannot be used for the effects assessment.